Literature summary extracted from

Vu, V.H.; Kim, K.
Improvement of cellulase activity using error-prone rolling circle amplification and site-directed mutagenesis (2012), J. Microbiol. Biotechnol., 22, 607-613.

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.1.4	E289V	mutation identified by error-prone rolling circle amplification. Mutation results in a 7.93-fold increase in its enzymatic activity	Bacillus amyloliquefaciens
3.2.1.4	additional information	improvement of endoglucanase activity by utilizing error-prone rolling circle amplification, supplemented with 1.7 mM MnCl2. The procedure generates random mutations in the Bacillus amyloliquefaciens endoglucanase gene with a frequency of 10 mutations per kilobase	Bacillus amyloliquefaciens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.4	Bacillus amyloliquefaciens	-	-	-
3.2.1.4	Bacillus amyloliquefaciens DL-3	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2.1.4	0.7	-	wild-type, pH 4.8, 50°C	Bacillus amyloliquefaciens
3.2.1.4	5.55	-	mutant E289V, pH 4.8, 55°C	Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.4	carboxymethyl cellulose + H2O	-	Bacillus amyloliquefaciens	?	-	?
3.2.1.4	carboxymethyl cellulose + H2O	-	Bacillus amyloliquefaciens DL-3	?	-	?

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Release 2023.1 (January 2023)