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Literature summary extracted from
- Purification and characterization of beta-glucosidase from Weissella cibaria 37 (2012), J. Microbiol. Biotechnol., 22, 1705-1713.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expressed in Escherichia coli BL21(DE3) cells | Weissella cibaria |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Cu2+ | 5 mM Cu2+ reduces the activity to 46.1% | Weissella cibaria |  |
| 3.2.1.21 | E-64 | 1 mM E-64 reduces the activity to 78.3% | Weissella cibaria | |
| 3.2.1.21 | Fe3+ | 5 mM Fe3+ reduces the activity to 55.8% | Weissella cibaria | |
| 3.2.1.21 | Mn2+ | 5 mM Mn2+ reduces the activity to 86.9% | Weissella cibaria | |
| 3.2.1.21 | additional information | not inhibited by EDTA and phenylmethylsulfonyl fluoride | Weissella cibaria | |
| 3.2.1.21 | pepstatin | 1 mM pepstatin reduces the activity to 45.2% | Weissella cibaria | |
| 3.2.1.21 | SDS | 1 mM SDS reduces the activity to 16.9% | Weissella cibaria | |
| 3.2.1.21 | Zn2+ | 5 mM Cu2+ reduces the activity to 56.7% | Weissella cibaria | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 4.04 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 5.5 and 45°C | Weissella cibaria | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Ca2+ | 5 mM Ca2+ slightly increases the activity to 108.2% | Weissella cibaria | |
| 3.2.1.21 | additional information | not influenced by Co2+, K+, and Mg2+ | Weissella cibaria |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 49951 | - |
x * 49951, calculated from amino acid sequence | Weissella cibaria |
| 3.2.1.21 | 50000 | - |
x * 50000, SDS-PAGE | Weissella cibaria |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Weissella cibaria | I6TNE2 | - |
- |
| 3.2.1.21 | Weissella cibaria 37 | I6TNE2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | Ni+2-nitrilotriacetic acid column chromatography | Weissella cibaria |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Weissella cibaria | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Weissella cibaria 37 | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | carboxymethyl cellulose + H2O | - |
Weissella cibaria | ? | - |
? | |
| 3.2.1.21 | carboxymethyl cellulose + H2O | - |
Weissella cibaria 37 | ? | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Weissella cibaria | ? | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Weissella cibaria 37 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 50000, SDS-PAGE | Weissella cibaria |
| 3.2.1.21 | ? | x * 49951, calculated from amino acid sequence | Weissella cibaria |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | beta-D-glucoside glucohydrolase | - |
Weissella cibaria |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 45 | - |
- |
Weissella cibaria |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 30 | 55 | more than 50% activity between 30 and 52°C. Activity decreases rapidly at temperature above 55°C | Weissella cibaria |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | 50 | enzyme activity decreases rapidly during the first 10 min and then gradually decreases to about 30% until 60 min when incubated at 40-50°C | Weissella cibaria |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 30.63 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 5.5 and 45°C | Weissella cibaria | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.5 | - |
- |
Weissella cibaria |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | 7.5 | more than 50% activity between pH 5.0 and 7.5 | Weissella cibaria |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Weissella cibaria | calculated from amino acid sequence | - |
5.1 |
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