EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | EDTA | 114.7% activity at 10 mM | Sphingomonas sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expressed in Escherichia coli BL21(DE3) cells | Sphingomonas sp. |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.22 | the enzyme shows strong resistance to trypsin and proteinase K digestion. After treatment at 37°C for 1 h with trypsin and proteinase K, the enzyme retained 102.1% and 114% of its initial activity, respectively | Sphingomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag+ | complete inhibition at 10 mM | Sphingomonas sp. | |
3.2.1.22 | FeSO4 | 69.5% residual acitivty at 10 mM | Sphingomonas sp. | |
3.2.1.22 | Hg2+ | complete inhibition at 10 mM | Sphingomonas sp. | |
3.2.1.22 | Mg2+ | 93.4% residual activity at 10 mM | Sphingomonas sp. | |
3.2.1.22 | additional information | not inhibited by EDTA, 2-mercaptoethanol | Sphingomonas sp. | |
3.2.1.22 | SDS | 7.6% residual acitivty at 10 mM | Sphingomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 2.2 | - |
4-nitrophenyl alpha-D-galactopyranoside | at pH 5.0 and 60°C | Sphingomonas sp. | |
3.2.1.22 | 29.4 | - |
melibiose | at pH 5.0 and 60°C | Sphingomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ca2+ | 119.7% activity at 10 mM | Sphingomonas sp. | |
3.2.1.22 | Mn2+ | 110.8% activity at 10 mM | Sphingomonas sp. | |
3.2.1.22 | additional information | not influenced by Zn2+, Fe3+, K+, and Cu2+ | Sphingomonas sp. | |
3.2.1.22 | Na+ | 104.9% activity at 10 mM | Sphingomonas sp. | |
3.2.1.22 | Ni2+ | 113.7% activity at 10 mM | Sphingomonas sp. | |
3.2.1.22 | Pb2+ | 113.5% activity at 10 mM | Sphingomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 82300 | - |
x * 82300, calculated from amino acid sequence | Sphingomonas sp. |
3.2.1.22 | 85500 | - |
x * 85500, SDS-PAGE | Sphingomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | melibiose + H2O | Sphingomonas sp. | - |
D-galactose + D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | Sphingomonas sp. JB13 | - |
D-galactose + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Sphingomonas sp. | M4GRS1 | - |
- |
3.2.1.22 | Sphingomonas sp. JB13 | M4GRS1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | Ni-NTA agarose column chromatography | Sphingomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 1.7 | - |
with raffinose as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
3.2.1.22 | 2.8 | - |
with cottonseed meal as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
3.2.1.22 | 6.9 | - |
with soybean meal as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
3.2.1.22 | 221 | - |
with melibiose as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
3.2.1.22 | 225 | - |
with 4-nitrophenyl alpha-D-galactopyranoside as substrate, at pH 5.0 and 60°C | Sphingomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | 4-nitrophenyl alpha-D-galactopyranoside + H2O | best substrate | Sphingomonas sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
3.2.1.22 | 4-nitrophenyl alpha-D-galactopyranoside + H2O | best substrate | Sphingomonas sp. JB13 | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
3.2.1.22 | cottonseed meal + H2O | - |
Sphingomonas sp. | ? | - |
? | |
3.2.1.22 | cottonseed meal + H2O | - |
Sphingomonas sp. JB13 | ? | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Sphingomonas sp. | D-galactose + D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Sphingomonas sp. JB13 | D-galactose + D-glucose | - |
? | |
3.2.1.22 | raffinose + H2O | worst substrate | Sphingomonas sp. | sucrose + D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | worst substrate | Sphingomonas sp. JB13 | sucrose + D-galactose | - |
? | |
3.2.1.22 | soybean meal + H2O | - |
Sphingomonas sp. | ? | - |
? | |
3.2.1.22 | soybean meal + H2O | - |
Sphingomonas sp. JB13 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.22 | ? | x * 82300, calculated from amino acid sequence | Sphingomonas sp. |
3.2.1.22 | ? | x * 85500, SDS-PAGE | Sphingomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | 1,6-alpha-D-galactoside galactohydrolase | - |
Sphingomonas sp. |
3.2.1.22 | agaAJB13 | - |
Sphingomonas sp. |
3.2.1.22 | melibiase | - |
Sphingomonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 60 | - |
- |
Sphingomonas sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 30 | 65 | more than 50% activity between 30 and 65°C | Sphingomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 60 | - |
the enzyme has a half-life of more than 60 min at 60°C and retains about 25% activity at 20°C, and about 10% at 10°C. At 65°C and 70°C, the enzyme activity decreases rapidly | Sphingomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 448 | - |
melibiose | at pH 5.0 and 60°C | Sphingomonas sp. | |
3.2.1.22 | 512 | - |
4-nitrophenyl alpha-D-galactopyranoside | at pH 5.0 and 60°C | Sphingomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | - |
- |
Sphingomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.5 | 6.5 | more than 50% activity between pH 4.5 and 6.5 | Sphingomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4 | 11 | the enzyme exhibits more than 40% of its initial activity after incubation in buffers ranging from pH 4.0 to 11.0 at 37°C for 1 h | Sphingomonas sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.22 | Sphingomonas sp. | calculated from amino acid sequence | - |
5.2 |