BRENDA - Enzyme Database show

Cloning, characterization, and inhibition studies of a beta-carbonic anhydrase from Leishmania donovani chagasi, the protozoan parasite responsible for leishmaniasis

Syrjaenen, L.; Vermelho, A.B.; Rodrigues, I.d.e..A.; Corte-Real, S.; Salonen, T.; Pan, P.; Vullo, D.; Parkkila, S.; Capasso, C.; Supuran, C.T.; J. Med. Chem. 56, 7372-7381 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.1
using the baculovirus expression system as a His-tagged fusion protein
Leishmania chagasi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
(2Z)-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(3-bromobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(3-methoxybenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(4-fluorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(4-methylpentan-2-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-benzylidene-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
-
Leishmania chagasi
4.2.1.1
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
Leishmania chagasi
4.2.1.1
1,2-benzothiazol-3(2H)-one 1,1-dioxide
-
Leishmania chagasi
4.2.1.1
1-(1,2-benzoxazol-3-yl)methanesulfonamide
-
Leishmania chagasi
4.2.1.1
2-(hydroxymethyl)-4-nitro-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
-
Leishmania chagasi
4.2.1.1
3,5-dichloro-2-hydroxybenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
3-aminobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4,5-dichlorobenzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-Aminobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-hydroxybenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-sulfamoylbenzoic acid
-
Leishmania chagasi
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
5-([(4-aminophenyl)sulfonyl]amino)-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
-
Leishmania chagasi
4.2.1.1
6-ethoxy-1,3-benzothiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
acetazolamide
-
Leishmania chagasi
4.2.1.1
benzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
-
Leishmania chagasi
4.2.1.1
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
-
Leishmania chagasi
4.2.1.1
N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
-
Leishmania chagasi
4.2.1.1
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
-
Leishmania chagasi
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
0.0158
-
CO2
pH 7.5, 20°C
Leishmania chagasi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
35000
-
SDS-PAGE
Leishmania chagasi
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Leishmania chagasi
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
730164
Leishmania chagasi
H2CO3
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
935000
-
CO2
pH 7.5, 20°C
Leishmania chagasi
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.0000134
-
(2Z)-2-(3-bromobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000184
-
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000195
-
(2Z)-2-(4-methylpentan-2-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000279
-
(2Z)-2-benzylidene-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000331
-
(2Z)-2-(4-fluorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000401
-
(2Z)-2-(3-methoxybenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000502
-
2-(hydroxymethyl)-4-nitro-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000502
-
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000515
-
6-ethoxy-1,3-benzothiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000596
-
4-sulfamoylbenzoic acid
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000734
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000074
-
N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000871
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000871
-
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000917
-
acetazolamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000917
-
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000951
-
5-([(4-aminophenyl)sulfonyl]amino)-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000953
-
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000136
-
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000144
-
(2Z)-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000152
-
(2Z)-2-(5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000189
-
4,5-dichlorobenzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000227
-
4-(2-hydroxyethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000236
-
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000316
-
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000338
-
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000389
-
4-(hydroxymethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000433
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000584
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000705
-
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000764
-
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000806
-
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000834
-
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000927
-
4-hydroxybenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.004819
-
4-amino-6-chlorobenzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.00596
-
3-aminobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.00842
-
4-amino-3-bromobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.00891
-
benzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009058
-
4-amino-3-chlorobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009083
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009135
-
3,5-dichloro-2-hydroxybenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009251
-
4-Aminobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0156
-
4-amino-3-fluorobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
1-(1,2-benzoxazol-3-yl)methanesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
1,2-benzothiazol-3(2H)-one 1,1-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
4-(2-aminoethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
4-(aminomethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
using the baculovirus expression system as a His-tagged fusion protein
Leishmania chagasi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
(2Z)-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(3-bromobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(3-methoxybenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(4-fluorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(4-methylpentan-2-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-(5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(2Z)-2-benzylidene-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
-
Leishmania chagasi
4.2.1.1
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
Leishmania chagasi
4.2.1.1
1,2-benzothiazol-3(2H)-one 1,1-dioxide
-
Leishmania chagasi
4.2.1.1
1-(1,2-benzoxazol-3-yl)methanesulfonamide
-
Leishmania chagasi
4.2.1.1
2-(hydroxymethyl)-4-nitro-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
-
Leishmania chagasi
4.2.1.1
3,5-dichloro-2-hydroxybenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
3-aminobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4,5-dichlorobenzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-Aminobenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-hydroxybenzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-sulfamoylbenzoic acid
-
Leishmania chagasi
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
5-([(4-aminophenyl)sulfonyl]amino)-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
-
Leishmania chagasi
4.2.1.1
6-ethoxy-1,3-benzothiazole-2-sulfonamide
-
Leishmania chagasi
4.2.1.1
acetazolamide
-
Leishmania chagasi
4.2.1.1
benzene-1,3-disulfonamide
-
Leishmania chagasi
4.2.1.1
benzenesulfonamide
-
Leishmania chagasi
4.2.1.1
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
-
Leishmania chagasi
4.2.1.1
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
-
Leishmania chagasi
4.2.1.1
N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
-
Leishmania chagasi
4.2.1.1
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
-
Leishmania chagasi
4.2.1.1
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
-
Leishmania chagasi
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.0000134
-
(2Z)-2-(3-bromobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000184
-
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000195
-
(2Z)-2-(4-methylpentan-2-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000279
-
(2Z)-2-benzylidene-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000331
-
(2Z)-2-(4-fluorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000401
-
(2Z)-2-(3-methoxybenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000502
-
2-(hydroxymethyl)-4-nitro-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000502
-
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000515
-
6-ethoxy-1,3-benzothiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000596
-
4-sulfamoylbenzoic acid
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000734
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000074
-
N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000871
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000871
-
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000917
-
acetazolamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000917
-
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000951
-
5-([(4-aminophenyl)sulfonyl]amino)-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0000953
-
(2Z)-2-(4-chlorobenzylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000136
-
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000144
-
(2Z)-2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000152
-
(2Z)-2-(5-bromo-2-oxo-1,2-dihydro-3H-indol-3-ylidene)-N-(5-sulfanyl-1,3,4-thiadiazol-2-yl)hydrazinecarboxamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000189
-
4,5-dichlorobenzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000227
-
4-(2-hydroxyethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000236
-
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000316
-
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000338
-
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000389
-
4-(hydroxymethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000433
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000584
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000705
-
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000764
-
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000806
-
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000834
-
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.000927
-
4-hydroxybenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.004819
-
4-amino-6-chlorobenzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.00596
-
3-aminobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.00842
-
4-amino-3-bromobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.00891
-
benzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009058
-
4-amino-3-chlorobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009083
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009135
-
3,5-dichloro-2-hydroxybenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.009251
-
4-Aminobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.0156
-
4-amino-3-fluorobenzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
1-(1,2-benzoxazol-3-yl)methanesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
1,2-benzothiazol-3(2H)-one 1,1-dioxide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
4-(2-aminoethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
4-(aminomethyl)benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
4.2.1.1
0.1
-
benzenesulfonamide
pH 7.5, 20°C
Leishmania chagasi
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
0.0158
-
CO2
pH 7.5, 20°C
Leishmania chagasi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
35000
-
SDS-PAGE
Leishmania chagasi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
730164
Leishmania chagasi
H2CO3
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
935000
-
CO2
pH 7.5, 20°C
Leishmania chagasi
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
59000
-
CO2
pH 7.5, 20°C
Leishmania chagasi
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
59000
-
CO2
pH 7.5, 20°C
Leishmania chagasi