BRENDA - Enzyme Database show

DNA cloning, characterization, and inhibition studies of an alpha-carbonic anhydrase from the pathogenic bacterium Vibrio cholerae

Del Prete, S.; Isik, S.; Vullo, D.; De Luca, V.; Carginale, V.; Scozzafava, A.; Supuran, C.T.; Capasso, C.; J. Med. Chem. 55, 10742-10748 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.1
in Escherichia coli as a His-tagged-fusion protein
Vibrio cholerae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
-
Vibrio cholerae
4.2.1.1
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
Vibrio cholerae
4.2.1.1
1,2-benzothiazol-3(2H)-one 1,1-dioxide
-
Vibrio cholerae
4.2.1.1
1-(1,2-benzoxazol-3-yl)methanesulfonamide
-
Vibrio cholerae
4.2.1.1
2-aminobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
2-hydrazinylbenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
-
Vibrio cholerae
4.2.1.1
4,5-dichlorobenzene-1,3-disulfonamide
-
Vibrio cholerae
4.2.1.1
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-Aminobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-hydrazinylbenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-sulfamoylbenzoic acid
-
Vibrio cholerae
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
-
Vibrio cholerae
4.2.1.1
6-ethoxy-1,3-benzothiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
-
Vibrio cholerae
4.2.1.1
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
-
Vibrio cholerae
4.2.1.1
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
-
Vibrio cholerae
4.2.1.1
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
-
Vibrio cholerae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
11.7
-
CO2
pH 7.5, 20C
Vibrio cholerae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
26000
-
SDS-PAGE
Vibrio cholerae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.1
Vibrio cholerae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.1
using Ni-NTA chromatography
Vibrio cholerae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
730159
Vibrio cholerae
H2CO3
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
823000
-
CO2
pH 7.5, 20C
Vibrio cholerae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.00000059
-
5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.00000069
-
6-ethoxy-1,3-benzothiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000025
-
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000036
-
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000042
-
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000042
-
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000047
-
6-hydroxy-1,3-benzothiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000063
-
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000068
-
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000008
-
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000121
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000235
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000303
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000371
-
4,5-dichlorobenzene-1,3-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000427
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000453
-
4-amino-6-chlorobenzene-1,3-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000521
-
2-hydrazinylbenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000545
-
4-(hydroxymethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000567
-
4-(2-hydroxyethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000598
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000629
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000715
-
4-sulfamoylbenzoic acid
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000795
-
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000884
-
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000897
-
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000991
-
4-amino-3-iodobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000125
-
4-hydrazinylbenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000133
-
4-amino-3-bromobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000139
-
4-amino-3-chlorobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000199
-
4-amino-3-fluorobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000219
-
benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000402
-
4-(2-aminoethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.00044
-
2-aminobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000447
-
4-(aminomethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000471
-
4-Aminobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000982
-
1-(1,2-benzoxazol-3-yl)methanesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
1,2-benzothiazol-3(2H)-one 1,1-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
in Escherichia coli as a His-tagged-fusion protein
Vibrio cholerae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.1
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
-
Vibrio cholerae
4.2.1.1
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
-
Vibrio cholerae
4.2.1.1
1,2-benzothiazol-3(2H)-one 1,1-dioxide
-
Vibrio cholerae
4.2.1.1
1-(1,2-benzoxazol-3-yl)methanesulfonamide
-
Vibrio cholerae
4.2.1.1
2-aminobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
2-hydrazinylbenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
-
Vibrio cholerae
4.2.1.1
4,5-dichlorobenzene-1,3-disulfonamide
-
Vibrio cholerae
4.2.1.1
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(2-aminoethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(2-hydroxyethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(aminomethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-(hydroxymethyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-bromobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-chlorobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-fluorobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-3-iodobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-6-chlorobenzene-1,3-disulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-Aminobenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-hydrazinylbenzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-sulfamoylbenzoic acid
-
Vibrio cholerae
4.2.1.1
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
5-amino-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
-
Vibrio cholerae
4.2.1.1
6-ethoxy-1,3-benzothiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
6-hydroxy-1,3-benzothiazole-2-sulfonamide
-
Vibrio cholerae
4.2.1.1
benzenesulfonamide
-
Vibrio cholerae
4.2.1.1
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
-
Vibrio cholerae
4.2.1.1
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
-
Vibrio cholerae
4.2.1.1
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
-
Vibrio cholerae
4.2.1.1
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
-
Vibrio cholerae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.1
0.00000059
-
5-(3-chlorophenyl)-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.00000069
-
6-ethoxy-1,3-benzothiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000025
-
(4R)-4-(ethylamino)-2-(3-methoxypropyl)-3,4-dihydro-2H-thieno[3,2-e][1,2]thiazine-6-sulfonamide 1,1-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000036
-
N-[(2E)-3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene]acetamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000042
-
4-amino-N-(4-sulfamoylphenyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000042
-
5-[(phenylsulfonyl)amino]-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000047
-
6-hydroxy-1,3-benzothiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000063
-
(4S,6S)-4-(ethylamino)-6-methyl-5,6-dihydro-4H-thieno[2,3-b]thiopyran-2-sulfonamide 7,7-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000068
-
N-(5-sulfamoyl-1,3,4-thiadiazol-2-yl)acetamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000008
-
N-(3-chloro-1H-indol-7-yl)benzene-1,4-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000121
-
5-imino-4-methyl-4,5-dihydro-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000235
-
5-amino-1,3,4-thiadiazole-2-sulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000303
-
4-amino-N-[2-(4-sulfamoylphenyl)ethyl]benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000371
-
4,5-dichlorobenzene-1,3-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000427
-
4-amino-N-(4-sulfamoylbenzyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000453
-
4-amino-6-chlorobenzene-1,3-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000521
-
2-hydrazinylbenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000545
-
4-(hydroxymethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000567
-
4-(2-hydroxyethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000598
-
4-[(2-aminopyrimidin-4-yl)amino]benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000629
-
4-amino-6-(trifluoromethyl)benzene-1,3-disulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000715
-
4-sulfamoylbenzoic acid
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000795
-
6-chloro-3,4-dihydro-2H-1,2,4-benzothiadiazine-7-sulfonamide 1,1-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000884
-
4-(1,1-dioxido-1,2-thiazinan-2-yl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000897
-
4-(5-methyl-3-phenyl-1,2-oxazol-4-yl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.0000991
-
4-amino-3-iodobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000125
-
4-hydrazinylbenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000133
-
4-amino-3-bromobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000139
-
4-amino-3-chlorobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000199
-
4-amino-3-fluorobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000219
-
benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000402
-
4-(2-aminoethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.00044
-
2-aminobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000447
-
4-(aminomethyl)benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000471
-
4-Aminobenzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.000982
-
1-(1,2-benzoxazol-3-yl)methanesulfonamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
1,2-benzothiazol-3(2H)-one 1,1-dioxide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
2-methoxy-N-[(1-methylpyrrolidin-2-yl)methyl]-5-sulfamoylbenzamide
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
[(3aS,5aR,8aR,8bS)-2,2,7,7-tetramethyltetrahydro-3aH-bis[1,3]dioxolo[4,5-b:4',5'-d]pyran-3a-yl]methyl sulfamate
pH 7.5, 20C
Vibrio cholerae
4.2.1.1
0.001
-
4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide
pH 7.5, 20C
Vibrio cholerae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.1
11.7
-
CO2
pH 7.5, 20C
Vibrio cholerae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.1
26000
-
SDS-PAGE
Vibrio cholerae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.1
using Ni-NTA chromatography
Vibrio cholerae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.1
CO2 + H2O
-
730159
Vibrio cholerae
H2CO3
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.1
823000
-
CO2
pH 7.5, 20C
Vibrio cholerae
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
70000
-
CO2
pH 7.5, 20C
Vibrio cholerae
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.1
70000
-
CO2
pH 7.5, 20C
Vibrio cholerae