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Literature summary extracted from

  • Gong, Y.Y.; Yin, X.; Zhang, H.M.; Wu, M.C.; Tang, C.D.; Wang, J.Q.; Pang, Q.F.
    Cloning, expression of a feruloyl esterase from Aspergillus usamii E001 and its applicability in generating ferulic acid from wheat bran (2013), J. Ind. Microbiol. Biotechnol., 40, 1433-1441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 gene AufaeA, subcloning in Escherichia coli strains DH5alpha and JM109, expression in Pichia pastoris strain GS115, the enzyme is secreted Aspergillus usamii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 additional information
-
 additional information Michaelis-Menten kinetics Aspergillus usamii
3.1.1.73 4.64
-
 methyl ferulate pH 5.0, 45°C Aspergillus usamii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.73 additional information the enzyme activity is not significantly affected by EDTA and metal ions tested, i.e. Na+, Li+, Ca2+, Co2+, Ba2+, Fe2+, Mg2+, Cu2+, Mn2+, Sn2+, Zn2+, Fe3+, Al3+ Aspergillus usamii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.73 28300
-
 x * 36000, recombinant enzyme, SDS-PAGE, x * 28300, about, sequence calculation Aspergillus usamii
3.1.1.73 36000
-
 x * 36000, recombinant enzyme, SDS-PAGE, x * 28300, about, sequence calculation Aspergillus usamii

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus usamii
-
 isolated from the soil in China, gene AufaeA, type-A feruloyl esterase
-
3.1.1.73 Aspergillus usamii E001
-
 isolated from the soil in China, gene AufaeA, type-A feruloyl esterase
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.73 glycoprotein one putative N-linked glycosylation site in the AuFaeA sequence Aspergillus usamii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.73 recombinnat extracellular enzyme from Pichia pastoris strain GS115 by ammonium sulfate fractionation, dialysis, and ultrafiltration Aspergillus usamii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus usamii methanol + ferulate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus usamii E001 methanol + ferulate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.73 ? x * 36000, recombinant enzyme, SDS-PAGE, x * 28300, about, sequence calculation Aspergillus usamii

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 AuFaeA
-
 Aspergillus usamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 45
-
 recombinant enzyme Aspergillus usamii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.73 30 60 activity range Aspergillus usamii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 45
-
 purified recombinant enzyme, highly stable below Aspergillus usamii
3.1.1.73 50
-
 purified recombinant enzyme, loss of 45% activity after 60 min Aspergillus usamii
3.1.1.73 55
-
 purified recombinant enzyme, loss of 50% activity after 5 min, inactivation after 40 min Aspergillus usamii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
 recombinant enzyme Aspergillus usamii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.73 4 6.5 purified recombinant enzyme, highly stable within this range Aspergillus usamii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.73 Aspergillus usamii sequence calculation
-
 4.3

General Information

EC Number General Information Comment Organism
3.1.1.73 additional information the enzyme shows a synergistic interaction with the Aspergillus usamii GH family 11 xylanase A. Three-dimensional structure determination, modeling, overview. The enzyme has a globular shape with a catalytic triad Ser133-Asp194-His247, and is composed mainly of one major nine-stranded beta-sheet, two minor two-stranded beta-sheets, and seven alpha-helices, core topology is alpha/beta-hydrolase fold Aspergillus usamii