Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yuan, P.; Meng, K.; Shi, P.; Luo, H.; Huang, H.; Tu, T.; Yang, P.; Yao, B.
    An alkaline-active and alkali-stable pectate lyase from Streptomyces sp. S27 with potential in textile industry (2012), J. Ind. Microbiol. Biotechnol., 39, 909-915.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 gene pl-str, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), subcloning in Escherichia coli strain JM109 Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Co2+ 8% activation at 1 mM, 78.5% inhibition at 5 mM Streptomyces sp.
4.2.2.2 Cr3+ inhibits completely at 1 mM Streptomyces sp.
4.2.2.2 Cu2+ 11% activation at 1 mM, 41% inhibition at 5 mM Streptomyces sp.
4.2.2.2 EDTA inhibits completely at 1 mM Streptomyces sp.
4.2.2.2 Fe3+ 4% inhibition at 1 mM, 99% inhibition at 5 mM Streptomyces sp.
4.2.2.2 Li+ 5% activation at 1 mM, 62% inhibition at 5 mM Streptomyces sp.
4.2.2.2 Mg2+ 9% activation at 1 mM, 22% inhibition at 5 mM Streptomyces sp.
4.2.2.2 Ni2+ 4% inhibition at 1 mM, 93% inhibition at 5 mM Streptomyces sp.
4.2.2.2 Pb2+ 31% inhibition at 1 mM, complete inhibition at 5 mM Streptomyces sp.
4.2.2.2 Zn2+ 13% activation at 1 mM, 46% inhibition at 5 mM Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required, optimal at about 0.05 mM Streptomyces sp.
4.2.2.2 K+ 9% activation at 1 mM Streptomyces sp.
4.2.2.2 Mn2+ 29% activation at 1 mM, 7.5% activation at 5 mM Streptomyces sp.
4.2.2.2 Na+ 10% activation at 1 mM Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 33500
-
 x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Streptomyces sp.
4.2.2.2 35000
-
 x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Streptomyces sp.
-
 isolated from soil of Flaming Mountain in the Turpan Basin of Xinjiang, China, gene pl-str
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatgraphy Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 citrus pectin P9311
-
 Streptomyces sp. ?
-
 ?
4.2.2.2 citrus pectin P9436
-
 Streptomyces sp. ?
-
 ?
4.2.2.2 citrus pectin P9561
-
 Streptomyces sp. ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Streptomyces sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 PL-STR
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 60
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 37
-
 purified recombinant His-tagged enzyme, 1 h, without substrate, pH 12.0, over 58% activity remaining Streptomyces sp.
4.2.2.2 37
-
 purified recombinant His-tagged enzyme, 1 h, without substrate, pH 7.0-10.0, over 80% activity remaining Streptomyces sp.
4.2.2.2 37 50 purified recombinant His-tagged enzyme, 45 min, without substrate, pH 9.0-12.0, over 80% activity remaining Streptomyces sp.
4.2.2.2 50
-
 purified recombinant His-tagged enzyme, 45 min, without substrate, pH 10.0, over 80% activity remaining Streptomyces sp.
4.2.2.2 60
-
 purified recombinant His-tagged enzyme, 45 min, without substrate, pH 10.0, 30% activity remaining Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 10
-
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 7 10 purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain Streptomyces sp.
4.2.2.2 9 12 purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain Streptomyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Streptomyces sp. sequence calculation
-
 6.83

General Information

EC Number General Information Comment Organism
4.2.2.2 evolution PL-STR belongs to family PF00544 Streptomyces sp.