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Literature summary extracted from

  • Groisillier, A.; Shao, Z.; Michel, G.; Goulitquer, S.; Bonin, P.; Krahulec, S.; Nidetzky, B.; Duan, D.; Boyen, C.; Tonon, T.
    Mannitol metabolism in brown algae involves a new phosphatase family (2014), J. Exp. Bot., 65, 559-570.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.22 expressed in Escherichia coli BL21(DE3) cells Ectocarpus siliculosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.22 4-hydroxymercuribenzoate complete inhibition at 1 mM Ectocarpus siliculosus
3.1.3.22 Sodium fluoride complete inhibition at 0.1 mM Ectocarpus siliculosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.22 0.67
-
 D-mannitol 1-phosphate at pH 7.5 and 30°C Ectocarpus siliculosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.22 Mg2+ required, 5 mM used in assay conditions Ectocarpus siliculosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.22 36000
-
 4 * 36000, calculated from amino acid sequence Ectocarpus siliculosus
3.1.3.22 155000
-
 isoform M1Pase2, gel filtration Ectocarpus siliculosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.22 D-mannitol 1-phosphate + H2O Ectocarpus siliculosus highly specific substrate D-mannitol + phosphate
-
 ?
3.1.3.22 D-mannitol 1-phosphate + H2O Ectocarpus siliculosus CCAP 1310/4 highly specific substrate D-mannitol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.22 Ectocarpus siliculosus
-
-
-
3.1.3.22 Ectocarpus siliculosus CCAP 1310/4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.22 Ni2+-affinity column chromatography Ectocarpus siliculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.22 D-fructose 1-phosphate + H2O low activity Ectocarpus siliculosus D-fructose + phosphate
-
 ?
3.1.3.22 D-fructose 1-phosphate + H2O low activity Ectocarpus siliculosus CCAP 1310/4 D-fructose + phosphate
-
 ?
3.1.3.22 D-mannitol 1-phosphate + H2O highly specific substrate Ectocarpus siliculosus D-mannitol + phosphate
-
 ?
3.1.3.22 D-mannitol 1-phosphate + H2O highly specific substrate Ectocarpus siliculosus CCAP 1310/4 D-mannitol + phosphate
-
 ?
3.1.3.22 additional information no activity with D-mannose 6-phosphate, D-fructose 6-phosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate Ectocarpus siliculosus ?
-
 ?
3.1.3.22 additional information no activity with D-mannose 6-phosphate, D-fructose 6-phosphate, D-glucose 1-phosphate, and D-glucose 6-phosphate Ectocarpus siliculosus CCAP 1310/4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.22 homotetramer 4 * 36000, calculated from amino acid sequence Ectocarpus siliculosus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.22 M1Pase2
-
 Ectocarpus siliculosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.22 0.015
-
 D-mannitol 1-phosphate at pH 7.5 and 30°C Ectocarpus siliculosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.22 0.02239
-
 D-mannitol 1-phosphate at pH 7.5 and 30°C Ectocarpus siliculosus