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Literature summary extracted from

  • Chen, X.; Chen, G.; Truksa, M.; Snyder, C.L.; Shah, S.; Weselake, R.J.
    Glycerol-3-phosphate acyltransferase 4 is essential for the normal development of reproductive organs and the embryo in Brassica napus (2014), J. Exp. Bot., 65, 4201-4215.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.B13 endoplasmic reticulum
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 Brassica napus 5783
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Organism

EC Number Organism UniProt Comment Textmining
2.3.1.198 Brassica napus H9XTI6 and H9XTI4 and H9XTI5 H9XTI6, i.e. isoform Gpat4-A1, H9XTI4 i.e. isoform Gpat4-C1, H9XTI5, i.e. isoform Gpat4-C2
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3.1.3.B13 Brassica napus
-
-
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3.1.3.B13 Brassica napus DH12075
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.198 additional information all three Gpat4 isoforms exhibit sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested Brassica napus ?
-
 ?
2.3.1.198 sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
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 Brassica napus CoA + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate reaction mainly occurs in sn-2 position. Intrinsic phosphatase activity of the enzymes finally leads to formation of 2-(15-carboxypentadecanoyl)-sn-glycerol ?
2.3.1.198 sn-glycerol 3-phosphate + palmitoyl-CoA
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 Brassica napus CoA + 2-palmitoyl-sn-glycerol 3-phosphate phosphatase activity of the enzymes does not act on non-substituted acyl residues ?
3.1.3.B13 additional information enzyme exhibits sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested Brassica napus ?
-
 ?
3.1.3.B13 additional information enzyme exhibits sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested Brassica napus DH12075 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.198 GPAT4
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 Brassica napus
3.1.3.B13 GPAT4
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 Brassica napus

General Information

EC Number General Information Comment Organism
2.3.1.198 physiological function RNAi-mediated down-regulation of all Gpat4 homologues causes abnormal development of several reproductive organs and reduced seed set. Both pollen grains and developing embryo sacs of the downregulated Gpat4 lines are affected. The gpat4 mature embryos show decreased cutin content and altered monomer composition. The defective embryo development affects the oil body morphology, oil content, and fatty acid composition in Gpat4-lacking seeds Brassica napus
3.1.3.B13 physiological function enzyme exhibits sn-2 acyltransferase and phosphatase activities with dicarboxylic acid-CoA as acyl donor. When non-substituted acyl-CoA is used as acyl donor, the rate of acylation is considerably lower and phosphatase activity is not manifested. RNA interference-mediated down-regulation of all isoform GPAT4 homologues under the control of the napin promoter causes abnormal development of several reproductive organs and reduced seed set. Both pollen grains and developing embryo sacs are affected. The gpat4 mature embryos show decreased cutin content and altered monomer composition, the defect further affects the oil body morphology, oil content, and fatty acid composition in gpat4 seeds Brassica napus