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Literature summary extracted from

  • Green, S.A.; Chen, X.; Nieuwenhuizen, N.J.; Matich, A.J.; Wang, M.Y.; Bunn, B.J.; Yauk, Y.K.; Atkinson, R.G.
    Identification, functional characterization, and regulation of the enzyme responsible for floral (E)-nerolidol biosynthesis in kiwifruit (Actinidia chinensis) (2012), J. Exp. Bot., 63, 1951-1967.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.48 0.0008
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis
4.2.3.48 0.0019
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.48 cytoplasm
-
 Actinidia chinensis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.48 Mg2+ Km value 0.117 mM Actinidia chinensis
4.2.3.48 Mn2+ Km value 0.014 mM Actinidia chinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.48 65400
-
 x * 65400, calculated Actinidia chinensis

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.48 Actinidia chinensis H9M5U5
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.48 flower
-
 Actinidia chinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.48 (2E,6E)-farnesyl diphosphate + H2O
-
 Actinidia chinensis (3S,6E)-nerolidol + diphosphate predominant enantiomer produced ?
4.2.3.48 geranyl diphosphate + H2O 58% of the velocity with (2E,6E)-farnesyl diphosphate Actinidia chinensis (S)-linalool + diphosphate
-
 ?
4.2.3.48 geranylgeranyl diphosphate + H2O 10% of the velocity with (2E,6E)-farnesyl diphosphate Actinidia chinensis (E,E)-geranyllinalool + diphosphate
-
 ?
4.2.3.48 additional information no substrate: dimethylallyl diphosphate Actinidia chinensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.48 ? x * 65400, calculated Actinidia chinensis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.48 NES1
-
 Actinidia chinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.48 0.03
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mn2+ Actinidia chinensis
4.2.3.48 0.13
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis
4.2.3.48 0.24
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis

Expression

EC Number Organism Comment Expression
4.2.3.48 Actinidia chinensis peak expression of enzyme correlates with peak (E)-nerolidol, but not linalool accumulation in flowers additional information

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.48 69
-
 geranyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis
4.2.3.48 300
-
 (2E,6E)-farnesyl diphosphate pH 7.5, 22°C, presence of Mg2+ Actinidia chinensis