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Literature summary extracted from

  • Giordana, L.; Mantilla, B.S.; Santana, M.; Silber, A.M.; Nowicki, C.
    Cystathionine gamma-lyase, an enzyme related to the reverse transsulfuration pathway, is functional in Leishmania spp. (2014), J. Eukaryot. Microbiol., 61, 204-213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.1 gene LmjF35.3230 or Lmj_CGL, functional complementation of a CGL null mutant strain DELTA CYS3 of Saccharomyces cerevisiae Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.1 DL-propargylglycine an irreversible enzyme inhibitor, molecular mechanism of DL-propargylglycine action in vivo Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.1 cytosol
-
 Leishmania mexicana 5829
-
4.4.1.1 cytosol
-
 Leishmania major 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.1 L-cystathionine + H2O Leishmania mexicana
-
 L-cysteine + 2-oxobutanoate + NH3
-
 r
4.4.1.1 L-cystathionine + H2O Leishmania major
-
 L-cysteine + 2-oxobutanoate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.1 Leishmania major E9AFE7 gene LmjF35.3230 or Lmj_CGL
-
4.4.1.1 Leishmania mexicana
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.1 promastigote
-
 Leishmania mexicana
-
4.4.1.1 promastigote
-
 Leishmania major
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.1 0.49
-
 cell-free extract of promastigotes, pH 8.0, 37°C Leishmania mexicana
4.4.1.1 0.49
-
 cell-free extract of promastigotes, pH 8.0, 37°C Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.1 L-cystathionine + H2O
-
 Leishmania mexicana L-cysteine + 2-oxobutanoate + NH3
-
 r
4.4.1.1 L-cystathionine + H2O
-
 Leishmania major L-cysteine + 2-oxobutanoate + NH3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.4.1.1 cgl
-
 Leishmania mexicana
4.4.1.1 cgl
-
 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.1 37
-
 assay at Leishmania mexicana
4.4.1.1 37
-
 assay at Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.1 8
-
 assay at Leishmania mexicana
4.4.1.1 8
-
 assay at Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.1 pyridoxal 5'-phosphate
-
 Leishmania mexicana
4.4.1.1 pyridoxal 5'-phosphate
-
 Leishmania major

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.4.1.1 0.065
-
 pH 7.4, 37°C Leishmania major DL-propargylglycine

General Information

EC Number General Information Comment Organism
4.4.1.1 additional information the Leishmania major enzyme is able to rescue the wild-type phenotype of a Saccharomyces cerevisiae enzyme-null mutant Leishmania major