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Literature summary extracted from

  • Liu, S.P.; Liu, R.X.; El-Rotail, A.A.; Ding, Z.Y.; Gu, Z.H.; Zhang, L.; Shi, G.Y.
    Heterologous pathway for the production of L-phenylglycine from glucose by E. coli (2014), J. Biotechnol., 186C, 91-97.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.46 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor
1.1.3.46 expressed in Escherichia coli BL21(DE3) cells Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.46 (S)-4-hydroxymandelate + O2 Streptomyces coelicolor
-
 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2 Amycolatopsis orientalis
-
 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2 Streptomyces coelicolor CGMCC 4.1092
-
 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2 Amycolatopsis orientalis ATCC 19795
-
 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.46 Amycolatopsis orientalis
-
-
-
1.1.3.46 Amycolatopsis orientalis ATCC 19795
-
-
-
1.1.3.46 Streptomyces coelicolor
-
-
-
1.1.3.46 Streptomyces coelicolor CGMCC 4.1092
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.46 HisTrap affinity column chromatography Streptomyces coelicolor
1.1.3.46 HisTrap affinity column chromatography Amycolatopsis orientalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.46 310
-
 pH and temperature not specified in the publication Amycolatopsis orientalis
1.1.3.46 1822
-
 pH and temperature not specified in the publication Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.46 (S)-4-hydroxymandelate + O2
-
 Streptomyces coelicolor 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2
-
 Amycolatopsis orientalis 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2
-
 Streptomyces coelicolor CGMCC 4.1092 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?
1.1.3.46 (S)-4-hydroxymandelate + O2
-
 Amycolatopsis orientalis ATCC 19795 2-(4-hydroxyphenyl)-2-oxoacetate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.46 HmO
-
 Streptomyces coelicolor
1.1.3.46 HmO
-
 Amycolatopsis orientalis
1.1.3.46 L-4-hydroxymandelate oxidase
-
 Streptomyces coelicolor
1.1.3.46 L-4-hydroxymandelate oxidase
-
 Amycolatopsis orientalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.46 50
-
-
 Streptomyces coelicolor
1.1.3.46 60
-
-
 Amycolatopsis orientalis