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Literature summary extracted from

  • Lindner, S.N.; Meiswinkel, T.M.; Panhorst, M.; Youn, J.W.; Wiefel, L.; Wendisch, V.F.
    Glycerol-3-phosphatase of Corynebacterium glutamicum (2012), J. Biotechnol., 159, 216-224.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.21 dithiothreitol the addition of at least 1 mM dithiothreitol is necessary to retain enzymatic activity during the assay Corynebacterium glutamicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.21 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.21 (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-2,4-dihydroxytetrahydro-2H-pyran-2-carboxylic acid
-
 Corynebacterium glutamicum
3.1.3.21 (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-4-hydroxy-2-methoxytetrahydro-2H-pyran-2-carboxylic acid
-
 Corynebacterium glutamicum
3.1.3.21 dimethyl (6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosidonate
-
 Corynebacterium glutamicum
3.1.3.21 disodium [(6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosonato-kO2](dioxido)oxovanadate(2-)
-
 Corynebacterium glutamicum
3.1.3.21 phosphate
-
 Corynebacterium glutamicum
3.1.3.21 [(3R,4R)-4-[(4R,6R,7R,8S)-7-(acetylamino)-8-hydroxy-3-oxo-1,2,5-trioxaspiro[3.5]non-6-yl]-3,4-dihydroxybutyl]phosphonic acid
-
 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.21 2.9
-
 L-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum
3.1.3.21 3.8
-
 DL-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.21 Mg2+ essential for activity, the enzyme prefers Mg2+ over Mn2+, 10 mM Mg2+ used in assay conditions Corynebacterium glutamicum
3.1.3.21 Mn2+ essential for activity Corynebacterium glutamicum
3.1.3.21 additional information Ca2+ does not stimulate enzyme activity Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.21 58600
-
 2 * 58600, calculated from amino acid sequence Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.21 D-glycerol 3-phosphate + H2O Corynebacterium glutamicum the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate glycerol + phosphate
-
 ?
3.1.3.21 D-glycerol 3-phosphate + H2O Corynebacterium glutamicum ATCC 13032 the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate glycerol + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O Corynebacterium glutamicum
-
 glycerol + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O Corynebacterium glutamicum ATCC 13032
-
 glycerol + phosphate
-
 ?
3.1.3.21 L-glycerol 3-phosphate + H2O Corynebacterium glutamicum
-
 glycerol + phosphate
-
 ?
3.1.3.21 L-glycerol 3-phosphate + H2O Corynebacterium glutamicum ATCC 13032
-
 glycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.21 Corynebacterium glutamicum
-
-
-
3.1.3.21 Corynebacterium glutamicum ATCC 13032
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.21
-
 Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.21 D-glycerol 3-phosphate + H2O the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate Corynebacterium glutamicum glycerol + phosphate
-
 ?
3.1.3.21 D-glycerol 3-phosphate + H2O the enzyme shows a preference for the D-enantiomer of glycerol 3-phosphate Corynebacterium glutamicum ATCC 13032 glycerol + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum glycerol + phosphate
-
 ?
3.1.3.21 DL-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum ATCC 13032 glycerol + phosphate
-
 ?
3.1.3.21 L-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum glycerol + phosphate
-
 ?
3.1.3.21 L-glycerol 3-phosphate + H2O
-
 Corynebacterium glutamicum ATCC 13032 glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.21 homodimer 2 * 58600, calculated from amino acid sequence Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
3.1.3.21 glycerol-3-phosphatase
-
 Corynebacterium glutamicum
3.1.3.21 GPP
-
 Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.21 25 37 the enzyme is stable at 25°C, at temperatures above 25°C activity decreases continuously with 45% and 15% activity remaining after 1 h of incubation at 30°C and 37°C, respectively Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.21 1.7
-
 L-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum
3.1.3.21 30.3
-
 DL-glycerol 3-phosphate at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.21 7.5
-
-
 Corynebacterium glutamicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.21 7 8.9 10% of enzyme activity remains at pH 7.0 and 33% at pH 8.9 Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.21 2.4
-
 phosphate with L-glycerol-3-phosphate as substrate, at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum
3.1.3.21 2.5
-
 phosphate with DL-glycerol-3-phosphate as substrate, at 30°C, pH 7.5, in 100 mM HEPES Corynebacterium glutamicum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.3.21 0.0065
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum disodium [(6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosonato-kO2](dioxido)oxovanadate(2-)
3.1.3.21 0.011
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-2,4-dihydroxytetrahydro-2H-pyran-2-carboxylic acid
3.1.3.21 0.0498
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum (2S,4S,5R,6R)-5-(acetylamino)-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-4-hydroxy-2-methoxytetrahydro-2H-pyran-2-carboxylic acid
3.1.3.21 0.0937
-
 in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum dimethyl (6R)-5-(acetylamino)-3,5-dideoxy-6-[(1R,2R)-1,2-dihydroxy-4-phosphonobutyl]-alpha-L-threo-hex-2-ulopyranosidonate
3.1.3.21 0.1
-
 IC50 above 0.1 mM, in 25 mM HEPES pH 7.5, 25 mM KCl, 1 mM MgCl2, 1 mM dithiothreitol, at 30°C Corynebacterium glutamicum [(3R,4R)-4-[(4R,6R,7R,8S)-7-(acetylamino)-8-hydroxy-3-oxo-1,2,5-trioxaspiro[3.5]non-6-yl]-3,4-dihydroxybutyl]phosphonic acid