BRENDA - Enzyme Database show

Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia

Joo, Y.C.; Seo, E.S.; Kim, Y.S.; Kim, K.R.; Park, J.B.; Oh, D.K.; J. Biotechnol. 158, 17-23 (2012)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.53
Tween 40
maximum activity at 0.05% (v/v) Tween 40
Stenotrophomonas maltophilia
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.53
expressed in Escherichia coli ER2566, BL21(DE3), W3110, and MLK1067 cells
Stenotrophomonas maltophilia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.53
0.46
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.53
68000
-
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
4.2.1.53
136000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.53
oleate + H2O
Stenotrophomonas maltophilia
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
4.2.1.53
oleate + H2O
Stenotrophomonas maltophilia KCTC 1773
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.53
Stenotrophomonas maltophilia
-
-
-
4.2.1.53
Stenotrophomonas maltophilia KCTC 1773
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.53
His-Trap column chromatography
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.53
0.014
-
with myristoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
0.033
-
with gamma-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
0.54
-
with alpha-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
0.91
-
with linoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
1.95
-
with palmitoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
3.69
-
with oleate as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.53
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
linoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12(Z)-octadecenoic acid
-
-
-
?
4.2.1.53
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia
?
-
-
-
-
4.2.1.53
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia KCTC 1773
?
-
-
-
-
4.2.1.53
myristoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxymyristic acid
-
-
-
?
4.2.1.53
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
4.2.1.53
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia KCTC 1773
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
4.2.1.53
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-Hydroxypalmitic acid
-
-
-
?
4.2.1.53
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-Hydroxypalmitic acid
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.53
homodimer
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.53
35
-
-
Stenotrophomonas maltophilia
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.53
11.83
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.53
6.5
-
-
Stenotrophomonas maltophilia
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.53
additional information
the addition of FAD (0.01-0.2 mM) has no effect on the activity
Stenotrophomonas maltophilia
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.53
Tween 40
maximum activity at 0.05% (v/v) Tween 40
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.53
expressed in Escherichia coli ER2566, BL21(DE3), W3110, and MLK1067 cells
Stenotrophomonas maltophilia
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.2.1.53
additional information
the addition of FAD (0.01-0.2 mM) has no effect on the activity
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.53
0.46
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.53
68000
-
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
4.2.1.53
136000
-
gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.53
oleate + H2O
Stenotrophomonas maltophilia
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
4.2.1.53
oleate + H2O
Stenotrophomonas maltophilia KCTC 1773
highest specific activity
(R)-10-hydroxystearate
98% yield after 4 h
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.53
His-Trap column chromatography
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.2.1.53
0.014
-
with myristoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
0.033
-
with gamma-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
0.54
-
with alpha-linolenic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
0.91
-
with linoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
1.95
-
with palmitoleic acid as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
4.2.1.53
3.69
-
with oleate as substrate, at pH 6.5 and 35C
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.53
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
alpha-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-12,15(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
gamma-linolenic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-hydroxy-6,12(Z,Z)-octadecadienoic acid
-
-
-
?
4.2.1.53
linoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxy-12(Z)-octadecenoic acid
-
-
-
?
4.2.1.53
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia
?
-
-
-
-
4.2.1.53
additional information
no hydration activity for myristic acid, palmitic acid, stearic acid, arachidic acid, petroselinic acid, elaidic acid, vaccenic acid, conjugated linoleic acids, arachidonic acid, erucic ac, and nervonic acid
730082
Stenotrophomonas maltophilia KCTC 1773
?
-
-
-
-
4.2.1.53
myristoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-hydroxymyristic acid
-
-
-
?
4.2.1.53
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
4.2.1.53
oleate + H2O
highest specific activity
730082
Stenotrophomonas maltophilia KCTC 1773
(R)-10-hydroxystearate
98% yield after 4 h
-
-
?
4.2.1.53
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia
10-Hydroxypalmitic acid
-
-
-
?
4.2.1.53
Palmitoleic acid + H2O
-
730082
Stenotrophomonas maltophilia KCTC 1773
10-Hydroxypalmitic acid
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.53
homodimer
2 * 68000, SDS-PAGE
Stenotrophomonas maltophilia
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.2.1.53
35
-
-
Stenotrophomonas maltophilia
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.2.1.53
11.83
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.53
6.5
-
-
Stenotrophomonas maltophilia
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.53
25.67
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.2.1.53
25.67
-
oleate
at pH 6.5 and 35C
Stenotrophomonas maltophilia