EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.41 | additional information | construction of beta-lactamase (AmpC) and cephalosporin acetyl esterase (Aes) single or double knockout mutant strains from Escherichia coli strains JM105 and JM109(DE3), the chromosomal genes ampC and aes are disrupted by the phage lambda red recombinase system, which involves three stepswith three helper plasmids pKD13, pKD46 and pCP20, single or double knockout of ampC and aes from Escherichia coli JM105 have minimal effects on formal growth of the recombinant cells. Recombinant expression of Pseudomonas sp. strain SE83 cephalosporin C acylase II (sCPCAcy), EC 3.5.1.93, from vector pMKC-sCPCacy restores the cephalosporin C decomposition activity to an even higher level compared to wild-type, overview. CPC acylase II catalyzes a one-step enzymatic conversion of cephalosporin C into 7-aminocephalosporanic acid | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.41 | cephalosporin C + H2O | Escherichia coli | - |
deacetylcephalosporin C + acetate | - |
? | |
3.1.1.41 | cephalosporin C + H2O | Escherichia coli JM105 | - |
deacetylcephalosporin C + acetate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.41 | Escherichia coli | - |
gene aes | - |
3.1.1.41 | Escherichia coli JM105 | - |
gene aes | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.41 | cephalosporin C + H2O | - |
Escherichia coli | deacetylcephalosporin C + acetate | - |
? | |
3.1.1.41 | cephalosporin C + H2O | - |
Escherichia coli JM105 | deacetylcephalosporin C + acetate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.41 | Aes | - |
Escherichia coli |