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Literature summary extracted from

  • Rodrigues, J.R.; Couto, A.; Cabezas, A.; Pinto, R.M.; Ribeiro, J.M.; Canales, J.; Costas, M.J.; Cameselle, J.C.
    Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements (2014), J. Biol. Chem., 289, 10620-10636.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.15 expressed in Escherichia coli BL21 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.28 C404A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.7.1.28 H221A inactive Homo sapiens
2.7.1.28 K204A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.7.1.28 S446A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens
2.7.1.28 T112A the mutant shows reduced activity compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.15 ATP competitive inhibitor Homo sapiens
4.6.1.15 additional information not inhibited by glycerol Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.28 0.00574
-
 ATP mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0071
-
 D-glyceraldehyde mutant enzyme S446A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0099
-
 dihydroxyacetone mutant enzyme S446A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0137
-
 D-glyceraldehyde mutant enzyme T112A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.015
-
 D-glyceraldehyde mutant enzyme K204A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0155
-
 dihydroxyacetone wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0162
-
 D-glyceraldehyde mutant enzyme C404A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0181
-
 D-glyceraldehyde wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0191
-
 dihydroxyacetone mutant enzyme C404A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0252
-
 dihydroxyacetone mutant enzyme K204A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0352
-
 ATP mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0432
-
 ATP wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0624
-
 ATP wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0637
-
 ATP mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0663
-
 dihydroxyacetone mutant enzyme T112A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0696
-
 ATP mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0806
-
 ATP mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0853
-
 ATP mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.0996
-
 ATP mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.183
-
 ATP mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.28 Mn2+ required Homo sapiens
4.6.1.15 Mn2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.28 ATP + D-glyceraldehyde Homo sapiens
-
 ADP + D-glyceraldehyde 3-phosphate
-
 ?
2.7.1.28 ATP + dihydroxyacetone Homo sapiens
-
 ADP + dihydroxyacetone phosphate
-
 ?
4.6.1.15 FAD Homo sapiens
-
 AMP + riboflavin cyclic-4',5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.28 Homo sapiens Q3LXA3
-
-
4.6.1.15 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.28 ATP + D-glyceraldehyde
-
 Homo sapiens ADP + D-glyceraldehyde 3-phosphate
-
 ?
2.7.1.28 ATP + dihydroxyacetone
-
 Homo sapiens ADP + dihydroxyacetone phosphate
-
 ?
2.7.1.28 additional information no activity with glycolaldehyde and glycerol Homo sapiens ?
-
 ?
4.6.1.15 FAD
-
 Homo sapiens AMP + riboflavin cyclic-4',5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.28 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.28 dAK
-
 Homo sapiens
2.7.1.28 dAK the gene encodes triokinase and FMN cyclase Homo sapiens
2.7.1.28 TKFC
-
 Homo sapiens
4.6.1.15 dAK
-
 Homo sapiens
4.6.1.15 FMN cyclase
-
 Homo sapiens
4.6.1.15 TKFC
-
 Homo sapiens
4.6.1.15 triokinase/FMN cyclase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.28 0.13
-
 dihydroxyacetone mutant enzyme S446A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.15
-
 ATP mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.16
-
 D-glyceraldehyde mutant enzyme T112A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.16
-
 ATP mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.5
-
 ATP mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.51
-
 dihydroxyacetone mutant enzyme T112A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.66
-
 ATP mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.74
-
 D-glyceraldehyde mutant enzyme S446A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 1.7
-
 ATP mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.09
-
 D-glyceraldehyde mutant enzyme K204A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.1
-
 ATP mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.17
-
 ATP mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.26
-
 dihydroxyacetone mutant enzyme C404A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.27
-
 D-glyceraldehyde mutant enzyme C404A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.34
-
 ATP mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.79
-
 dihydroxyacetone mutant enzyme K204A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 3.94
-
 ATP wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 4.59
-
 ATP wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 4.83
-
 D-glyceraldehyde wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 5
-
 dihydroxyacetone wild type enzyme, at pH 7.5 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.28 0.19
-
 ATP mutant enzyme T112A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 0.64
-
 ATP mutant enzyme T112A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 1.15
-
 ATP mutant enzyme C404A, with D-glyceraldehyde as cosubstrate,at pH 7.5 and 37°C Homo sapiens
2.7.1.28 1.2
-
 D-glyceraldehyde mutant enzyme T112A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 1.9
-
 ATP mutant enzyme S446A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.18
-
 ATP mutant enzyme C404A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.4
-
 ATP mutant enzyme K204A, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 2.6
-
 ATP mutant enzyme S446A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 4.2
-
 ATP mutant enzyme K204A, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 6.3
-
 ATP wild type enzyme, with D-glyceraldehyde as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 7.6
-
 dihydroxyacetone mutant enzyme T112A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 10.4
-
 D-glyceraldehyde mutant enzyme S446A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 10.5
-
 ATP wild type enzyme, with dihydroxyacetone as cosubstrate, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 13.1
-
 dihydroxyacetone mutant enzyme S446A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 14
-
 D-glyceraldehyde mutant enzyme C404A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 14.2
-
 D-glyceraldehyde mutant enzyme K204A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 27.5
-
 D-glyceraldehyde wild type enzyme, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 115
-
 dihydroxyacetone mutant enzyme K204A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 118
-
 dihydroxyacetone mutant enzyme C404A, at pH 7.5 and 37°C Homo sapiens
2.7.1.28 321
-
 dihydroxyacetone wild type enzyme, at pH 7.5 and 37°C Homo sapiens