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Literature summary extracted from

  • Wiegmann, E.M.; Westendorf, E.; Kalus, I.; Pringle, T.H.; Luebke, T.; Dierks, T.
    Arylsulfatase K, a novel lysosomal sulfatase (2013), J. Biol. Chem., 288, 30019-30028.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.1 expression in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.1 C80A about 20% of wild-type activity at neutral pH, about 5% of wild-type activity at pH optimum Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 phosphate
-
 Homo sapiens
3.1.6.1 sulfate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.1 10.9
-
 4-nitrocatechol sulfate pH 4.6, 37°C Homo sapiens
3.1.6.1 20.6
-
 4-nitrocatechol sulfate pH 4.6, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.1 lysosome protein carries mannose 6-phosphate, indicating lysosomal sorting Homo sapiens 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.1 68000
-
 x * 68000, SDS-PAGE of recombinant glycoprotein, cellular form Homo sapiens
3.1.6.1 70000
-
 x * 70000, SDS-PAGE of recombinant glycoprotein, secreted form Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Homo sapiens Q6UWY0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.6.1 glycoprotein when expressed in human cells, isoform ArsK is detected as a 68-kDa glycoprotein carrying at least four N-glycans of both the complex and high-mannose type. Protein carries mannose 6-phosphate, indicating lysosomal sorting Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.1 additional information mRNA expression is found in all tissues tested Homo sapiens
-
3.1.6.1 pancreas high level of expression Homo sapiens
-
3.1.6.1 placenta high level of expression Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 4-nitrocatechol sulfate + H2O
-
 Homo sapiens 4-nitrocatechol + sulfate
-
 ?
3.1.6.1 4-nitrophenyl sulfate + H2O
-
 Homo sapiens 4-nitrophenol + sulfate
-
 ?
3.1.6.1 additional information activity is dependent on residue cysteine80, which undergoes conversion to formylglycine. No substrate: 4-methylumbelliferyl sulfate Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.6.1 ? x * 68000, SDS-PAGE of recombinant glycoprotein, cellular form Homo sapiens
3.1.6.1 ? x * 70000, SDS-PAGE of recombinant glycoprotein, secreted form Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.6.1 ARSK
-
 Homo sapiens
3.1.6.1 arylsulfatase K
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.1 4.6
-
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.6.1 2.4
-
 pH 4.6, 37°C Homo sapiens phosphate
3.1.6.1 2.9
-
 pH 4.6, 37°C Homo sapiens sulfate