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Literature summary extracted from

  • Novellasdemunt, L.; Tato, I.; Navarro-Sabate, A.; Ruiz-Meana, M.; Mendez-Lucas, A.; Perales, J.C.; Garcia-Dorado, D.; Ventura, F.; Bartrons, R.; Rosa, J.L.
    Akt-dependent activation of the heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) isoenzyme by amino acids (2013), J. Biol. Chem., 288, 10640-10651.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.105 additional information amino acids increase beta-D-fructose 2,6-bisphosphate concentration in HeLa and in MCF-7 human cells, molecular mechanisms involved differ from those observed with insulin. Only essential amino acids without glutamine are required to activate the PI3K/mTORC2 and p38/MAPKAPK-2 pathways and to increase the beta-D-fructose 2,6-bisphosphate concentration. The observed effects in signaling and metabolism are produced by a mixture of essential amino acids Homo sapiens
2.7.1.105 additional information amino acids increase beta-D-fructose 2,6-bisphosphate concentration in primary cardiomyocytes, molecular mechanisms involved differ from those observed with insulin. Only essential amino acids without glutamine are required to activate the PI3K/mTORC2 and p38/MAPKAPK-2 pathways and to increase the beta-D-fructose 2,6-bisphosphate concentration. The observed effects in signaling and metabolism are produced by a mixture of essential amino acids Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.105 gene PFKFB2 Homo sapiens
2.7.1.105 gene PFKFB2 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.105 Mg2+ required Homo sapiens
2.7.1.105 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate Homo sapiens
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate Rattus norvegicus
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate Rattus norvegicus Sprague-Dawley
-
 ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O Rattus norvegicus
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O Homo sapiens
-
 D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.105 Homo sapiens O60825 gene/isozyme PFKFB2
-
2.7.1.105 Rattus norvegicus Q9JJH5 gene/isozyme PFKFB2
-
2.7.1.105 Rattus norvegicus Sprague-Dawley Q9JJH5 gene/isozyme PFKFB2
-
3.1.3.46 Homo sapiens O60825
-
-
3.1.3.46 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.105 phosphoprotein specific phosphorylation of heart 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoenzyme PFKFB2 at Ser483. This activation is mediated by the phosphatidylinositol-4,5-bisphosphate 3-kinase and p38 signaling pathways. Amino acid-activated Akt phosphorylates PFKFB2 at Ser483. Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Homo sapiens
2.7.1.105 phosphoprotein specific phosphorylation of heart 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase isoenzyme PFKFB2 at Ser483. This activation is mediated by the phosphatidylinositol-4,5-bisphosphate 3-kinase and p38 signaling pathways. Amino acid-activated Akt phosphorylates PFKFB2 at Ser483. Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Rattus norvegicus
3.1.3.46 phosphoprotein Akt phosphorylates the enzyme at Ser-483 Rattus norvegicus
3.1.3.46 phosphoprotein Akt phosphorylates the enzyme at Ser-483 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.105 cardiomyocyte primary Rattus norvegicus
-
2.7.1.105 heart heart isozyme PFKFB2 Homo sapiens
-
2.7.1.105 heart heart isozyme PFKFB2 Rattus norvegicus
-
2.7.1.105 HeLa cell
-
 Homo sapiens
-
2.7.1.105 MCF-7 cell
-
 Homo sapiens
-
3.1.3.46 cardiomyocyte
-
 Rattus norvegicus
-
3.1.3.46 heart
-
 Rattus norvegicus
-
3.1.3.46 heart
-
 Homo sapiens
-
3.1.3.46 HEK-293 cell
-
 Homo sapiens
-
3.1.3.46 HeLa cell
-
 Homo sapiens
-
3.1.3.46 MCF-7 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Homo sapiens ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
2.7.1.105 ATP + beta-D-fructose 6-phosphate
-
 Rattus norvegicus Sprague-Dawley ADP + beta-D-fructose 2,6-bisphosphate
-
 ?
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O
-
 Homo sapiens D-fructose 6-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.105 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Homo sapiens
2.7.1.105 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Rattus norvegicus
2.7.1.105 PFKFB2
-
 Homo sapiens
2.7.1.105 PFKFB2
-
 Rattus norvegicus
3.1.3.46 heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Rattus norvegicus
3.1.3.46 heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
-
 Homo sapiens
3.1.3.46 PFKFB2
-
 Rattus norvegicus
3.1.3.46 PFKFB2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.105 37
-
 assay at Homo sapiens
2.7.1.105 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.105 7.5 8 assay at Homo sapiens
2.7.1.105 7.5 8 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.105 ATP
-
 Homo sapiens
2.7.1.105 ATP
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.1.105 malfunction Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Homo sapiens
2.7.1.105 malfunction Akt inactivation blocks PFKFB2 phosphorylation and fructose 2,6-bisphosphate production Rattus norvegicus