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Literature summary extracted from

  • Kaneiwa, T.; Miyazaki, A.; Kogawa, R.; Mizumoto, S.; Sugahara, K.; Yamada, S.
    Identification of amino acid residues required for the substrate specificity of human and mouse chondroitin sulfate hydrolase (conventional hyaluronidase-4) (2012), J. Biol. Chem., 287, 42119-42128.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 expressed in COS-7 cells Mus musculus
3.2.1.35 expressed in COS-7 cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.35 0.5
-
 chondroitin sulfate D at pH 4.5 and 37°C Mus musculus
3.2.1.35 0.66
-
 chondroitin sulfate A at pH 4.5 and 37°C Mus musculus
3.2.1.35 2.16
-
 chondroitin sulfate C at pH 4.5 and 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Homo sapiens
-
-
-
3.2.1.35 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 embryo
-
 Mus musculus
-
3.2.1.35 placenta
-
 Homo sapiens
-
3.2.1.35 skeletal muscle
-
 Homo sapiens
-
3.2.1.35 testis
-
 Mus musculus
-
3.2.1.35 testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 chondroitin sulfate A + H2O
-
 Mus musculus ?
-
 ?
3.2.1.35 chondroitin sulfate A + H2O poor substrate Homo sapiens ?
-
 ?
3.2.1.35 chondroitin sulfate C + H2O
-
 Mus musculus ?
-
 ?
3.2.1.35 chondroitin sulfate C + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.35 chondroitin sulfate D + H2O
-
 Mus musculus ?
-
 ?
3.2.1.35 chondroitin sulfate D + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.35 additional information the enzyme depolymerizes various chondroitin sulfate isoforms to a similar extent, suggesting broad substrate specificity Mus musculus ?
-
 ?
3.2.1.35 additional information the enzyme strongly prefers D-glucuronic acid-(2-O-sulfate)-GalNAc(6-O-sulfate)-containing sequences typical in chondroitin sulfate D Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 chondroitin sulfate hydrolase
-
 Mus musculus
3.2.1.35 chondroitin sulfate hydrolase
-
 Homo sapiens
3.2.1.35 HYAL4
-
 Mus musculus
3.2.1.35 HYAL4
-
 Homo sapiens
3.2.1.35 hyaluronidase-4
-
 Mus musculus
3.2.1.35 hyaluronidase-4
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 4.5
-
-
 Mus musculus
3.2.1.35 4.5 5
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.2.1.35 physiological function the enzyme play a special role in the testes of adult mice being involved in the maturation of sperm or during fertilization Mus musculus