Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Stehle, T.; Sreeramulu, S.; Loehr, F.; Richter, C.; Saxena, K.; Jonker, H.R.; Schwalbe, H.
    The apo-structure of the low molecular weight protein-tyrosine phosphatase A (MptpA) from Mycobacterium tuberculosis allows for better target-specific drug development (2012), J. Biol. Chem., 287, 34569-34582.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.48 expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.48 17500
-
 x * 17500, calculated from amino acid sequence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.48 [a protein]-tyrosine phosphate + H2O Mycobacterium tuberculosis
-
 [a protein]-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.48 Mycobacterium tuberculosis P9WIA1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.48 phosphoprotein phosphorylation at Tyr128 and Tyr129 is catalyzed by the kinase PtkA Mycobacterium tuberculosis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.48 Ni-NTA column chromatography and Superdex 75 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis 4-nitrophenol + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Mycobacterium tuberculosis [a protein]-tyrosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.48 ? x * 17500, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.3.48 LMW-PTP
-
 Mycobacterium tuberculosis
3.1.3.48 low molecular weight protein-tyrosine phosphatase A
-
 Mycobacterium tuberculosis
3.1.3.48 MPtpA
-
 Mycobacterium tuberculosis
3.1.3.48 protein-tyrosine phosphatase
-
 Mycobacterium tuberculosis
3.1.3.48 PTP
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.1.3.48 physiological function low molecular weight protein-tyrosine phosphatase A is a key virulence factor of Mycobacterium tuberculosis, the enzyme is essential for infection of host macrophages Mycobacterium tuberculosis