Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.M.; Kiso, Y.; Muraki, T.; Kang, M.S.; Nakai, H.; Saburi, W.; Lang, W.; Kang, H.K.; Okuyama, M.; Mori, H.; Suzuki, R.; Funane, K.; Suzuki, N.; Momma, M.; Fujimoto, Z.; Oguma, T.; Kobayashi, M.; Kim, D.; Kimura, A.
    Novel dextranase catalyzing cycloisomaltooligosaccharide formation and identification of catalytic amino acids and their functions using chemical rescue approach (2012), J. Biol. Chem., 287, 19927-19935.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.11
-
 Paenibacillus sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.11 D1254N 36% of wild-type activity Paenibacillus sp.
3.2.1.11 D189A application of achemical rescue approach using alpha-isomaltotetraosyl fluoride with NaN3. Mutant forms small sized dextran from alpha-isomaltotetraosyl fluoride in the presence of NaN3 Paenibacillus sp.
3.2.1.11 D340G application of a chemical rescue approach using alpha-isomaltotetraosyl fluoride with NaN3. Mutant forms beta-isomaltotetraosyl azide indicating residue D340 as nucleophile catalyst Paenibacillus sp.
3.2.1.11 E412Q application of a chemical rescue approach using alpha-isomaltotetraosyl fluoride with NaN3. Mutant forms alpha-isomaltotetraosyl azide indicating residue E412 as acid/base catalyst Paenibacillus sp.
3.2.1.11 additional information a C-terminal truncated enzyme, residues Ala39-Ser1304, has 50% wild-type activity Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.11 0.000000183
-
 dextran T2000 pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 0.000288
-
 isomaltoheptaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 0.000386
-
 isomaltohexaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 0.0004
-
 isomaltopentaose pH 5.5, 35°C Paenibacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Paenibacillus sp. H2EI27
-
-
3.2.1.11 Paenibacillus sp. Dex2000 H2EI27
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Paenibacillus sp. isomaltotetraose + cycloisomaltooligosaccharides main products are isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14 ?
3.2.1.11 dextran T2000 + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.11 isomaltoheptaose + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.11 isomaltoheptaose + H2O
-
 Paenibacillus sp. Dex2000 ?
-
 ?
3.2.1.11 isomaltohexaose + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.11 isomaltohexaose + H2O
-
 Paenibacillus sp. Dex2000 ?
-
 ?
3.2.1.11 isomaltopentaose + H2O
-
 Paenibacillus sp. ?
-
 ?
3.2.1.11 isomaltopentaose + H2O
-
 Paenibacillus sp. Dex2000 ?
-
 ?
3.2.1.11 additional information glycosyl hydrolase family 66 enzymes may be classified into three types: dextranases showing only dextranolytic activity, dextranases catalyzing hydrolysis with low cyclization activity, and cycloisomaltooligosaccharide glucanotransferases showing cycloisomaltooligosaccharide-forming activity with low dextranolytic activity Paenibacillus sp. ?
-
 ?
3.2.1.11 additional information no or very low activity on substrates isomaltose, isomaltotriose, isomaltotetraose Paenibacillus sp. ?
-
 ?
3.2.1.11 additional information glycosyl hydrolase family 66 enzymes may be classified into three types: dextranases showing only dextranolytic activity, dextranases catalyzing hydrolysis with low cyclization activity, and cycloisomaltooligosaccharide glucanotransferases showing cycloisomaltooligosaccharide-forming activity with low dextranolytic activity Paenibacillus sp. Dex2000 ?
-
 ?
3.2.1.11 additional information no or very low activity on substrates isomaltose, isomaltotriose, isomaltotetraose Paenibacillus sp. Dex2000 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.11 4.36
-
 isomaltopentaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 7.53
-
 isomaltohexaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 7.93
-
 isomaltoheptaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 44.3
-
 dextran T2000 pH 5.5, 35°C Paenibacillus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.11 10500
-
 isomaltopentaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 19500
-
 isomaltohexaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 27500
-
 isomaltoheptaose pH 5.5, 35°C Paenibacillus sp.
3.2.1.11 242000000
-
 dextran T2000 pH 5.5, 35°C Paenibacillus sp.