EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.19 | 0.0014 | - |
[prokaryotic ubiquitin-like protein]-L-glutamate | 25°C, pH 7.4 | Mycobacterium tuberculosis | |
6.3.1.19 | 0.0087 | - |
ATP | 25°C, pH 7.4 | Mycobacterium tuberculosis | |
6.3.1.19 | 0.0142 | - |
[protein PanB]-L-lysine | 25°C, pH 7.4 | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.19 | Mycobacterium tuberculosis | P9WNU7 | - |
- |
6.3.1.19 | Mycobacterium tuberculosis ATCC 25618 | P9WNU7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.19 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.19 | ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein PanB]-L-lysine | [protein PanB]-L-lysine shows a 3 orders of magnitude stronger affinity than free lysine | Mycobacterium tuberculosis | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein PanB]-L-lysine | - |
? | |
6.3.1.19 | ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein PanB]-L-lysine | [protein PanB]-L-lysine shows a 3 orders of magnitude stronger affinity than free lysine | Mycobacterium tuberculosis ATCC 25618 | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein PanB]-L-lysine | - |
? | |
6.3.1.19 | ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | the enzyme catalyzes a two-step reaction mechanism proceeding through a gamma-glutamyl phosphate-mixed anhydride intermediate that is formed on the C-terminal glutamate of the prokaryotic ubiquitin-like protein (Pup) before transfer of Pup to the substrate acceptor lysine. Phosphorylated [prokaryotic ubiquitin-like protein]-L-glutamate and ADP are retained on the enzyme in a stable fashion following activation | Mycobacterium tuberculosis | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? | |
6.3.1.19 | ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [protein]-L-lysine | the enzyme catalyzes a two-step reaction mechanism proceeding through a gamma-glutamyl phosphate-mixed anhydride intermediate that is formed on the C-terminal glutamate of the prokaryotic ubiquitin-like protein (Pup) before transfer of Pup to the substrate acceptor lysine. Phosphorylated [prokaryotic ubiquitin-like protein]-L-glutamate and ADP are retained on the enzyme in a stable fashion following activation | Mycobacterium tuberculosis ATCC 25618 | ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[protein]-L-lysine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.19 | PafA | ambiguous | Mycobacterium tuberculosis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.19 | 25 | - |
assay at | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.19 | 7.4 | - |
assay at | Mycobacterium tuberculosis |