Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, S.; Grueschow, S.; Dordick, J.S.; Sherman, D.H.
    Molecular analysis of the role of tyrosine 224 in the active site of Streptomyces coelicolor RppA, a bacterial type III polyketide synthase (2007), J. Biol. Chem., 282, 12765-12772.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.233 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.233 C138A inactive with malonyl-CoA Streptomyces coelicolor
2.3.1.233 C138S inactive with malonyl-CoA Streptomyces coelicolor
2.3.1.233 Y224A the mutant shows 3% activity with malonyl-CoA compared to the wild type enzyme Streptomyces coelicolor
2.3.1.233 Y224C the mutant shows 50% activity with malonyl-CoA compared to the wild type enzyme Streptomyces coelicolor
2.3.1.233 Y224F the mutant shows 70% activity with malonyl-CoA compared to the wild type enzyme Streptomyces coelicolor
2.3.1.233 Y224G inactive with malonyl-CoA but capable of accepting acetoacetyl-CoA and acetyl-CoA Streptomyces coelicolor
2.3.1.233 Y224H inactive with malonyl-CoA but capable of accepting acetoacetyl-CoA and acetyl-CoA Streptomyces coelicolor
2.3.1.233 Y224L the mutant shows 65% activity with malonyl-CoA compared to the wild type enzyme Streptomyces coelicolor
2.3.1.233 Y224L the mutant shows strongly reduced catalytic efficiency with malonyl-CoA compared to the wild type enzyme Streptomyces griseus
2.3.1.233 Y224M the mutant shows 15% activity with malonyl-CoA compared to the wild type enzyme Streptomyces coelicolor
2.3.1.233 Y224S inactive with malonyl-CoA but capable of accepting acetoacetyl-CoA and acetyl-CoA Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.233 0.0011
-
malonyl-CoA wild type enzyme, at pH 7.4 and 30°C Streptomyces griseus
2.3.1.233 0.0039
-
malonyl-CoA wild type enzyme, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.0095
-
malonyl-CoA mutant enzyme Y224F, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.0111
-
malonyl-CoA mutant enzyme Y224L, at pH 7.4 and 30°C Streptomyces griseus
2.3.1.233 0.012
-
malonyl-CoA mutant enzyme Y224C, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.0171
-
malonyl-CoA mutant enzyme Y224M, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.0234
-
malonyl-CoA mutant enzyme Y224L, at pH 7.4 and 30°C Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.233 43000
-
x * 43000, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.233 5 malonyl-CoA Streptomyces coelicolor
-
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
?
2.3.1.233 5 malonyl-CoA Streptomyces griseus
-
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
?
2.3.1.233 5 malonyl-CoA Streptomyces coelicolor A3 (2)
-
1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.233 Streptomyces coelicolor
-
-
-
2.3.1.233 Streptomyces coelicolor A3 (2)
-
-
-
2.3.1.233 Streptomyces griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.233 nickel-nitrilotriacetic acid-agarose column chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.233 5 malonyl-CoA
-
Streptomyces coelicolor 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
?
2.3.1.233 5 malonyl-CoA
-
Streptomyces griseus 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
?
2.3.1.233 5 malonyl-CoA
-
Streptomyces coelicolor A3 (2) 1,3,6,8-tetrahydroxynaphthalene + 5 CoA + 5 CO2 + H2O
-
?
2.3.1.233 acetoacetyl-CoA
-
Streptomyces griseus 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O
-
?
2.3.1.233 acetoacetyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O
-
?
2.3.1.233 acetoacetyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor A3 (2) 4-hydroxy-6-methyl-2-pyrone + CoA + CO2 + H2O
-
?
2.3.1.233 acetyl-CoA
-
Streptomyces griseus ? + CoA + CO2 + H2O
-
?
2.3.1.233 acetyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor ? + CoA + CO2 + H2O
-
?
2.3.1.233 acetyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor A3 (2) ? + CoA + CO2 + H2O
-
?
2.3.1.233 benzoyl-CoA
-
Streptomyces griseus ? + CoA + CO2 + H2O
-
?
2.3.1.233 benzoyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor 4-hydroxy-6-phenyl-2H-pyran-2-one + CoA + CO2 + H2O
-
?
2.3.1.233 benzoyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor A3 (2) 4-hydroxy-6-phenyl-2H-pyran-2-one + CoA + CO2 + H2O
-
?
2.3.1.233 hexanoyl-CoA
-
Streptomyces griseus ? + CoA + CO2 + H2O
-
?
2.3.1.233 hexanoyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor 4-hydroxy-6-pentyl-2H-pyran-2-one + CoA + CO2 + H2O
-
?
2.3.1.233 hexanoyl-CoA + 2 malonyl-CoA
-
Streptomyces coelicolor A3 (2) 4-hydroxy-6-pentyl-2H-pyran-2-one + CoA + CO2 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
2.3.1.233 ? x * 43000, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
2.3.1.233 RppA
-
Streptomyces coelicolor
2.3.1.233 RppA
-
Streptomyces griseus
2.3.1.233 THN synthase
-
Streptomyces coelicolor
2.3.1.233 THN synthase
-
Streptomyces griseus
2.3.1.233 THNS
-
Streptomyces coelicolor
2.3.1.233 THNS
-
Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.233 0.0003
-
malonyl-CoA mutant enzyme Y224L, at pH 7.4 and 30°C Streptomyces griseus
2.3.1.233 0.002
-
malonyl-CoA mutant enzyme Y224M, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.004
-
malonyl-CoA mutant enzyme Y224L, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.005
-
malonyl-CoA mutant enzyme Y224C, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.005
-
malonyl-CoA wild type enzyme, at pH 7.4 and 30°C Streptomyces griseus
2.3.1.233 0.018
-
malonyl-CoA mutant enzyme Y224F, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.022
-
malonyl-CoA wild type enzyme, at pH 7.4 and 30°C Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.233 0.133
-
malonyl-CoA mutant enzyme Y224M, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.175
-
malonyl-CoA mutant enzyme Y224L, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 0.298
-
malonyl-CoA mutant enzyme Y224L, at pH 7.4 and 30°C Streptomyces griseus
2.3.1.233 0.396
-
malonyl-CoA mutant enzyme Y224C, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 1.93
-
malonyl-CoA mutant enzyme Y224F, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 5.74
-
malonyl-CoA wild type enzyme, at pH 7.4 and 30°C Streptomyces coelicolor
2.3.1.233 44.58
-
malonyl-CoA wild type enzyme, at pH 7.4 and 30°C Streptomyces griseus