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Literature summary extracted from

  • Thompson, T.B.; Katayama, K.; Watanabe, K.; Hutchinson, C.R.; Rayment, I.
    Structural and functional analysis of tetracenomycin F2 cyclase from Streptomyces glaucescens. A type II polyketide cyclase (2004), J. Biol. Chem., 279, 37956-37963.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.154 expressed in Escherichia coli BL21(DE3) cells Streptomyces glaucescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.154 macroseeding method, using 1.4 M (NH4)2SO4, 50 mM HEPES, pH 7.5 Streptomyces glaucescens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.154 D27N the mutant shows 14% activity compared to the wild type enzyme Streptomyces glaucescens
4.2.1.154 H26A the mutant shows 15% activity compared to the wild type enzyme Streptomyces glaucescens
4.2.1.154 H26Q the mutant shows 91% activity compared to the wild type enzyme Streptomyces glaucescens
4.2.1.154 H51A the mutant shows 16% activity compared to the wild type enzyme Streptomyces glaucescens
4.2.1.154 H51Q the mutant shows 96% activity compared to the wild type enzyme Streptomyces glaucescens
4.2.1.154 R40G the mutant shows 10% activity compared to the wild type enzyme Streptomyces glaucescens
4.2.1.154 R40K the mutant shows 16% activity compared to the wild type enzyme Streptomyces glaucescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.154 0.155
-
 tetracenomycin F2 wild type enzyme, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.191
-
 tetracenomycin F2 mutant enzyme H51Q, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.203
-
 tetracenomycin F2 mutant enzyme H26Q, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.732
-
 tetracenomycin F2 mutant enzyme H26A, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.816
-
 tetracenomycin F2 mutant enzyme R40K, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.892
-
 tetracenomycin F2 mutant enzyme H51A, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 1.05
-
 tetracenomycin F2 mutant enzymeD27N, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 1.2
-
 tetracenomycin F2 mutant enzyme R40G, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.154 tetracenomycin F2 Streptomyces glaucescens
-
 tetracenomycin F1 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.154 Streptomyces glaucescens P39890
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.154 Q-Sepharose column chromatography, phenyl-Sepharose column chromatography, and MonoQ column chromatography Streptomyces glaucescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.154 tetracenomycin F2
-
 Streptomyces glaucescens tetracenomycin F1 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.154 dimer
-
 Streptomyces glaucescens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.154 tcmI
-
 Streptomyces glaucescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.154 0.0146
-
 tetracenomycin F2 mutant enzyme R40G, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0192
-
 tetracenomycin F2 mutant enzyme H26A, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0198
-
 tetracenomycin F2 mutant enzymeD27N, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0219
-
 tetracenomycin F2 mutant enzyme H51A, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.025
-
 tetracenomycin F2 mutant enzyme R40K, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.131
-
 tetracenomycin F2 mutant enzyme H26Q, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.136
-
 tetracenomycin F2 mutant enzyme H51Q, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.142
-
 tetracenomycin F2 wild type enzyme, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.154 0.0121
-
 tetracenomycin F2 mutant enzyme R40G, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0188
-
 tetracenomycin F2 mutant enzymeD27N, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0245
-
 tetracenomycin F2 mutant enzyme H51A, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0262
-
 tetracenomycin F2 mutant enzyme H26A, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.0306
-
 tetracenomycin F2 mutant enzyme R40K, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.645
-
 tetracenomycin F2 mutant enzyme H26Q, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.712
-
 tetracenomycin F2 mutant enzyme H51Q, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens
4.2.1.154 0.916
-
 tetracenomycin F2 wild type enzyme, at 30°C in 0.1 M Tris-HCl, pH 7.5 Streptomyces glaucescens