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Literature summary extracted from

  • Mitsuya, D.; Tanaka, S.; Matsumura, H.; Urano, N.; Takano, K.; Ogasahara, K.; Takehira, M.; Yutani, K.; Ishida, M.
    Strategy for cold adaptation of the tryptophan synthase alpha subunit from the psychrophile Shewanella frigidimarina K14-2: crystal structure and physicochemical properties (2014), J. Biochem., 155, 73-82.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 expressed in Escherichia coli JM109 cells Shewanella frigidimarina

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.20 sitting drop vapor diffusion method, using 0.1M HEPES-Na, pH 7.5, 2% (v/v) polyethylene glycol 400 and 2 M ammonium sulfate, at 4°C Shewanella frigidimarina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate Shewanella frigidimarina
-
 L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate Shewanella frigidimarina K14-2
-
 L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Shewanella frigidimarina
-
-
-
4.2.1.20 Shewanella frigidimarina K14-2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.20 SuperQ-650M column chromatography, Sephacryl S-300HR column chromatography, and MonoQ HR5/5 column chromatography Shewanella frigidimarina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 1-C-(indol-3-yl)glycerol 3-phosphate
-
 Shewanella frigidimarina D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 1-C-(indol-3-yl)glycerol 3-phosphate
-
 Shewanella frigidimarina K14-2 D-glyceraldehyde 3-phosphate + indole
-
 ?
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate
-
 Shewanella frigidimarina L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate
-
 Shewanella frigidimarina K14-2 L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 tryptophan synthase alpha subunit
-
 Shewanella frigidimarina
4.2.1.20 TSA
-
 Shewanella frigidimarina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.20 42.5 45
-
 Shewanella frigidimarina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.20 56
-
 the denaturation temperature of the enzyme is 56°C at pH 7.0 Shewanella frigidimarina