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Literature summary extracted from
- A novel glucoamylase activated by manganese and calcium produced in submerged fermentation by Aspergillus phoenicis (2014), J. Basic Microbiol., 54, 333-339.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | Na2HPO4 | 5 mM, 120% of initial activity | Aspergillus phoenicis |  |
| 3.2.1.3 | NaCl | 5 mM, 117% of initial activity | Aspergillus phoenicis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | Ba2+ | 5 mM, 90% residual activity | Aspergillus phoenicis | |
| 3.2.1.3 | Fe3+ | 5 mM, 83% residual activity | Aspergillus phoenicis | |
| 3.2.1.3 | Mg2+ | 5 mM, 85% residual activity | Aspergillus phoenicis | |
| 3.2.1.3 | Pb2+ | 5 mM, 77% residual activity | Aspergillus phoenicis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.3 | Ca2+ | 5 mM, 230% of initial activity | Aspergillus phoenicis | |
| 3.2.1.3 | Co2+ | 5 mM, 131% of initial activity | Aspergillus phoenicis | |
| 3.2.1.3 | Mn2+ | 5 mM, 276% of initial activity | Aspergillus phoenicis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.3 | Aspergillus phoenicis | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.3 | starch + H2O | - |
Aspergillus phoenicis | D-glucose + ? | sole product | ? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 60 | 65 | - |
Aspergillus phoenicis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 30 | 65 | completely stable for 1 h | Aspergillus phoenicis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 4.5 | - |
sodium acetate buffer | Aspergillus phoenicis |
| 3.2.1.3 | 5 | - |
McIlvaine buffer | Aspergillus phoenicis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.3 | 3 | 6 | - |
Aspergillus phoenicis |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.3 | Aspergillus phoenicis | enzyme expression is inhibited by glucose | down |
| 3.2.1.3 | Aspergillus phoenicis | enzyme expression is induced by maltose | up |
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