Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Casabon, I.; Swain, K.; Crowe, A.M.; Eltis, L.D.; Mohn, W.W.
    Actinobacterial acyl coenzyme A synthetases involved in steroid side-chain catabolism (2014), J. Bacteriol., 196, 579-587.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.42 expressed in Escherichia coli Rosetta 2(DE3)/pLysS cells Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.42 ATP + 3-oxocholest-4-en-26-oate + CoA Mycobacterium tuberculosis
-
 AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA
-
 ?
6.2.1.42 ATP + 3-oxocholest-4-en-26-oate + CoA Mycobacterium tuberculosis H37Rv
-
 AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.42 Mycobacterium tuberculosis
-
-
-
6.2.1.42 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.42 Ni-Sepharose column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.42 ATP + 3-hydroxy-5-cholesten-26-oate
-
 Mycobacterium tuberculosis ?
-
 ?
6.2.1.42 ATP + 3-hydroxy-5-cholesten-26-oate
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?
6.2.1.42 ATP + 3-oxocholest-4-en-26-oate + CoA
-
 Mycobacterium tuberculosis AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA
-
 ?
6.2.1.42 ATP + 3-oxocholest-4-en-26-oate + CoA
-
 Mycobacterium tuberculosis H37Rv AMP + diphosphate + 3-oxocholest-4-en-26-oyl-CoA
-
 ?
6.2.1.42 additional information no activity with cholate, chenodeoxycholate, 3-oxo-23,24-bisnorchol-4-en-22-oic acid, 3-hydroxy-9-oxo-9,10-seco-23,24-bisnorchola-1,3,5(10)-trien-22-oate, 1beta(2'-propanoate)-3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-5-indanone, and 3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione Mycobacterium tuberculosis ?
-
 ?
6.2.1.42 additional information no activity with cholate, chenodeoxycholate, 3-oxo-23,24-bisnorchol-4-en-22-oic acid, 3-hydroxy-9-oxo-9,10-seco-23,24-bisnorchola-1,3,5(10)-trien-22-oate, 1beta(2'-propanoate)-3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-5-indanone, and 3aalpha-H-4alpha(3'-propanoate)-7abeta-methylhexahydro-1,5-indanedione Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.42 fadD19
-
 Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.42 33
-
 3-oxocholest-4-en-26-oate at pH 7.3 and 22°C Mycobacterium tuberculosis