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Literature summary extracted from

  • Takeo, M.; Ohara, A.; Sakae, S.; Okamoto, Y.; Kitamura, C.; Kato, D.; Negoro, S.
    Function of a glutamine synthetase-like protein in bacterial aniline oxidation via gamma-glutamylanilide (2013), J. Bacteriol., 195, 4406-4414.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.123 gene atdA2, recombinant expression in enzyme-deficient, non-catechol-degrading Pseudomonas putida strain KT2440 Acinetobacter sp.
6.3.1.18 expressed in Escherichia coli strain SO58 Acinetobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.18 Mg2+ required, 5 mM used in assay conditions Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.18 55000
-
 6 * 55000, SDS-PAGE Acinetobacter sp.
6.3.1.18 350000
-
 gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.123 additional information Acinetobacter sp. a recombinant Pseudomonas strain harboring only atdA2 hydrolyzes gamma-glutamate into aniline, reversing the gamma-glutamate formation by AtdA1, a glutamine synthetase (GS)-like protein, EC 6.3.1.18 ?
-
 ?
3.5.1.123 N5-phenyl-L-glutamine + H2O Acinetobacter sp.
-
 L-glutamate + aniline
-
 ?
6.3.1.18 ATP + L-glutamate + aniline Acinetobacter sp. 100% activity ADP + phosphate + N5-phenyl-L-glutamine
-
 ?
6.3.1.18 ATP + L-glutamate + aniline Acinetobacter sp. YAA 100% activity ADP + phosphate + N5-phenyl-L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.123 Acinetobacter sp.
-
-
-
3.5.1.123 Acinetobacter sp. YAA
-
-
-
6.3.1.18 Acinetobacter sp.
-
-
-
6.3.1.18 Acinetobacter sp. YAA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.18 Hi-Trap Q Sepharose column chromatography and Hi-Trap butyl FF column chromatography Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.123 additional information a recombinant Pseudomonas strain harboring only atdA2 hydrolyzes gamma-glutamate into aniline, reversing the gamma-glutamate formation by AtdA1, a glutamine synthetase (GS)-like protein, EC 6.3.1.18 Acinetobacter sp. ?
-
 ?
3.5.1.123 N5-phenyl-L-glutamine + H2O
-
 Acinetobacter sp. L-glutamate + aniline
-
 ?
6.3.1.18 ATP + L-glutamate + 2-chloroaniline 92% activity compared to aniline Acinetobacter sp. ?
-
 ?
6.3.1.18 ATP + L-glutamate + 2-chloroaniline 92% activity compared to aniline Acinetobacter sp. YAA ?
-
 ?
6.3.1.18 ATP + L-glutamate + 2-methylaniline 40% activity compared to aniline Acinetobacter sp. ?
-
 ?
6.3.1.18 ATP + L-glutamate + 2-methylaniline 40% activity compared to aniline Acinetobacter sp. YAA ?
-
 ?
6.3.1.18 ATP + L-glutamate + 3-chloroaniline 69% activity compared to aniline Acinetobacter sp. ?
-
 ?
6.3.1.18 ATP + L-glutamate + 3-chloroaniline 69% activity compared to aniline Acinetobacter sp. YAA ?
-
 ?
6.3.1.18 ATP + L-glutamate + 3-methylaniline 27% activity compared to aniline Acinetobacter sp. ?
-
 ?
6.3.1.18 ATP + L-glutamate + 3-methylaniline 27% activity compared to aniline Acinetobacter sp. YAA ?
-
 ?
6.3.1.18 ATP + L-glutamate + 4-chloroaniline 92% activity compared to aniline Acinetobacter sp. ?
-
 ?
6.3.1.18 ATP + L-glutamate + 4-methylaniline 45% activity compared to aniline Acinetobacter sp. ?
-
 ?
6.3.1.18 ATP + L-glutamate + aniline 100% activity Acinetobacter sp. ADP + phosphate + N5-phenyl-L-glutamine
-
 ?
6.3.1.18 ATP + L-glutamate + aniline 100% activity Acinetobacter sp. YAA ADP + phosphate + N5-phenyl-L-glutamine
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.18 homohexamer 6 * 55000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.123 atdA2
-
 Acinetobacter sp.
3.5.1.123 glutamine amidotransferase-like protein
-
 Acinetobacter sp.
6.3.1.18 atdA1
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.18 40
-
-
 Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.18 8 10
-
 Acinetobacter sp.

General Information

EC Number General Information Comment Organism
3.5.1.123 metabolism the enzyme, encoded by gene atdA2, is involved in aniline oxidation as a part of the aniline degradation gene cluster, catechol is the major oxidation product from aniline, gamma-glutamate is an intermediate to catechol and a direct substrate for the dioxygenase. Acinetobacter sp. strain YAA has an aniline degradation gene cluster on the plasmid pYA1, the cluster consists of 14 genes (atdA1 to atdA5 and atdRSBCDEFGH) required for the conversion of aniline into tricarboxylic acid cycle intermediates. Putative aniline oxidation mechanism of Acinetobacter sp. YAA, pathway overview Acinetobacter sp.
3.5.1.123 physiological function enzyme AtdA2 prevents gamma-glutamate accumulation that is harmful to the cell Acinetobacter sp.