Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hemker, M.; Stratmann, A.; Goeke, K.; Schröder, W.; Lenz, J.; Piepersberg, W.; Pape, H.
    Identification, cloning, expression, and characterization of the extracellular acarbose-modifying glycosyltransferase, AcbD, from Actinoplanes sp. strain SE50 (2001), J. Bacteriol., 183, 4484-4492.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.99.B5 heterologous expression in Streptomyces lividans TK23 Actinoplanes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.99.B5 EDTA
-
Actinoplanes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.99.B5 0.65
-
acarbose pH 6.3, 30°C Actinoplanes sp.
2.4.99.B5 0.96
-
maltose pH 6.3, 30°C Actinoplanes sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.99.B5 extracellular
-
Actinoplanes sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.99.B5 Ca2+ required Actinoplanes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.99.B5 76000
-
x * 76000, SDS-PAGE Actinoplanes sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.99.B5 acarbose + maltotriose Actinoplanes sp. the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds acarviosyl-alpha-(1-4)-maltotriose + maltose
-
?
2.4.99.B5 acarbose + maltotriose Actinoplanes sp. 50/110 the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds acarviosyl-alpha-(1-4)-maltotriose + maltose
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.99.B5 Actinoplanes sp. Q27GR5
-
-
2.4.99.B5 Actinoplanes sp. 50/110 Q27GR5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.99.B5
-
Actinoplanes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.99.B5 acarbose + cellobiose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-cellobiose + maltose
-
?
2.4.99.B5 acarbose + cellobiose
-
Actinoplanes sp. 50/110 acarviosyl-(1->4)-alpha-cellobiose + maltose
-
?
2.4.99.B5 acarbose + laminaribiose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
?
2.4.99.B5 acarbose + laminaribiose
-
Actinoplanes sp. 50/110 acarviosyl-(1->4)-alpha-laminaribiose + maltose
-
?
2.4.99.B5 acarbose + maltoheptaose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
?
2.4.99.B5 acarbose + maltoheptaose
-
Actinoplanes sp. 50/110 acarviosyl-(1->4)-alpha-maltoheptaose + maltose
-
?
2.4.99.B5 acarbose + maltohexaose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-maltohexaose + maltose
-
?
2.4.99.B5 acarbose + maltopentaose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-maltopentaose + maltose
-
?
2.4.99.B5 acarbose + maltose
-
Actinoplanes sp. acarviosyl-alpha-(1->4)-maltose + maltose
-
?
2.4.99.B5 acarbose + maltotetraose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-maltotetraose + maltose
-
?
2.4.99.B5 acarbose + maltotriose the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds Actinoplanes sp. acarviosyl-alpha-(1-4)-maltotriose + maltose
-
?
2.4.99.B5 acarbose + maltotriose the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds Actinoplanes sp. 50/110 acarviosyl-alpha-(1-4)-maltotriose + maltose
-
?
2.4.99.B5 acarbose + maltotriose
-
Actinoplanes sp. acarviosyl-alpha-(1->4)-maltotriose + maltose
-
?
2.4.99.B5 acarbose + nonyl alpha-D-glucopyranoside
-
Actinoplanes sp. nonyl acarviosyl-(1->4)-alpha-D-glucopyranoside + maltose
-
?
2.4.99.B5 acarbose + xylobiose
-
Actinoplanes sp. acarviosyl-(1->4)-alpha-xylobiose + maltose
-
?
2.4.99.B5 acarviosyl glucose + maltose
-
Actinoplanes sp. acarviosyl-alpha-(1->4)-maltose + D-glucose
-
?
2.4.99.B5 acarviosyl-alpha-(1->4)-Glc-alpha-(1->4)-Glc-alpha-(1->4)-alpha-Glc + maltose
-
Actinoplanes sp. acarviosyl-maltose + Glc-alpha-(1->4)-Glc-alpha-(1->4)-Glc
-
?
2.4.99.B5 additional information a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity Actinoplanes sp. ?
-
?
2.4.99.B5 additional information a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity Actinoplanes sp. 50/110 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.99.B5 ? x * 76000, SDS-PAGE Actinoplanes sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.99.B5 AcbD
-
Actinoplanes sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.99.B5 30
-
-
Actinoplanes sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.99.B5 40
-
thermostable up to 40°C Actinoplanes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.99.B5 6.2 6.9
-
Actinoplanes sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.99.B5 Actinoplanes sp. isoelectric focusing, pH gradient from 3 to 10
-
5