EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.99.B5 | heterologous expression in Streptomyces lividans TK23 | Actinoplanes sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.99.B5 | EDTA | - |
Actinoplanes sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.99.B5 | 0.65 | - |
acarbose | pH 6.3, 30°C | Actinoplanes sp. | |
2.4.99.B5 | 0.96 | - |
maltose | pH 6.3, 30°C | Actinoplanes sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.99.B5 | extracellular | - |
Actinoplanes sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.99.B5 | Ca2+ | required | Actinoplanes sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 76000 | - |
x * 76000, SDS-PAGE | Actinoplanes sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.B5 | acarbose + maltotriose | Actinoplanes sp. | the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds | acarviosyl-alpha-(1-4)-maltotriose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltotriose | Actinoplanes sp. 50/110 | the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds | acarviosyl-alpha-(1-4)-maltotriose + maltose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.99.B5 | Actinoplanes sp. | Q27GR5 | - |
- |
2.4.99.B5 | Actinoplanes sp. 50/110 | Q27GR5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.99.B5 | - |
Actinoplanes sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.99.B5 | acarbose + cellobiose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-cellobiose + maltose | - |
? | |
2.4.99.B5 | acarbose + cellobiose | - |
Actinoplanes sp. 50/110 | acarviosyl-(1->4)-alpha-cellobiose + maltose | - |
? | |
2.4.99.B5 | acarbose + laminaribiose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-laminaribiose + maltose | - |
? | |
2.4.99.B5 | acarbose + laminaribiose | - |
Actinoplanes sp. 50/110 | acarviosyl-(1->4)-alpha-laminaribiose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltoheptaose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-maltoheptaose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltoheptaose | - |
Actinoplanes sp. 50/110 | acarviosyl-(1->4)-alpha-maltoheptaose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltohexaose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-maltohexaose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltopentaose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-maltopentaose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltose | - |
Actinoplanes sp. | acarviosyl-alpha-(1->4)-maltose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltotetraose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-maltotetraose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltotriose | the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds | Actinoplanes sp. | acarviosyl-alpha-(1-4)-maltotriose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltotriose | the enzyme is involved in the extracellular formation of the series of acarbose-homologous compounds | Actinoplanes sp. 50/110 | acarviosyl-alpha-(1-4)-maltotriose + maltose | - |
? | |
2.4.99.B5 | acarbose + maltotriose | - |
Actinoplanes sp. | acarviosyl-alpha-(1->4)-maltotriose + maltose | - |
? | |
2.4.99.B5 | acarbose + nonyl alpha-D-glucopyranoside | - |
Actinoplanes sp. | nonyl acarviosyl-(1->4)-alpha-D-glucopyranoside + maltose | - |
? | |
2.4.99.B5 | acarbose + xylobiose | - |
Actinoplanes sp. | acarviosyl-(1->4)-alpha-xylobiose + maltose | - |
? | |
2.4.99.B5 | acarviosyl glucose + maltose | - |
Actinoplanes sp. | acarviosyl-alpha-(1->4)-maltose + D-glucose | - |
? | |
2.4.99.B5 | acarviosyl-alpha-(1->4)-Glc-alpha-(1->4)-Glc-alpha-(1->4)-alpha-Glc + maltose | - |
Actinoplanes sp. | acarviosyl-maltose + Glc-alpha-(1->4)-Glc-alpha-(1->4)-Glc | - |
? | |
2.4.99.B5 | additional information | a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity | Actinoplanes sp. | ? | - |
? | |
2.4.99.B5 | additional information | a wide range of additional donor and acceptor substrates are determined for the enzyme. Acceptors reveal a structural requirement for glucose-analogous structures conserving only the overall stereochemistry, except for the anomeric C atom, and the hydroxyl groups at positions 2, 3, and 4 of D-glucose. The enzyme shows neither alpha-amylase nor cyclodextrin glucanotransferase activity | Actinoplanes sp. 50/110 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.99.B5 | ? | x * 76000, SDS-PAGE | Actinoplanes sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.99.B5 | AcbD | - |
Actinoplanes sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 30 | - |
- |
Actinoplanes sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 40 | - |
thermostable up to 40°C | Actinoplanes sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.99.B5 | 6.2 | 6.9 | - |
Actinoplanes sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.4.99.B5 | Actinoplanes sp. | isoelectric focusing, pH gradient from 3 to 10 | - |
5 |