EC Number | Application | Comment | Organism |
---|---|---|---|
2.5.1.86 | drug development | the enzyme is a target for development of specific inhibitors for anti-infective therapies that block virulence factor formation, the enzyme is a target in treatment of tuberculosis | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.86 | gene Rv2361c, recombinant expression | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.86 | in complex with BPH-640, hanging drop vapor diffusion method, using | Mycobacterium tuberculosis |
2.5.1.86 | purified recombinant enzyme in complex with inhibitor BPH-640, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution | Mycobacterium tuberculosis |
2.5.1.153 | native enzyme and complexes with inhibitor BPH-629, and mutants Y51F with TPP, mutant Y51F/Y90F with TPP, to 1.8-2.7 A resolution | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.153 | D34A | compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol | Mycobacterium tuberculosis |
2.5.1.153 | R38A | compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol | Mycobacterium tuberculosis |
2.5.1.153 | Y51F | production of both (13S)-isotuberculosinol and tuberculosinol is reduced | Mycobacterium tuberculosis |
2.5.1.153 | Y51F/Y90F | no enzymic activity | Mycobacterium tuberculosis |
2.5.1.153 | Y90F | compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 49% tuberculosinol | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.86 | BPH-640 | a bisphosphonate inhibitor, three-dimensional structure from crystal structure, dimeric DPPS structure with one bound BPH-640 molecule in each monomer, overview. BPH-640 occupies a dimer interface binding site, sandwiched between residues G77, N78, G79, R80, T83, R89, and R127 of monomer A and residues R292 and F293 of monomer B | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.86 | cell wall | - |
Mycobacterium tuberculosis | 5618 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.86 | Mg2+ | required | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Mycobacterium tuberculosis | - |
7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | Mycobacterium tuberculosis H37Rv | - |
7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate | Mycobacterium tuberculosis | - |
diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate | Mycobacterium tuberculosis H37Rv | - |
diphosphate + trans,octacis-decaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.86 | Mycobacterium tuberculosis | P9WFF7 | - |
- |
2.5.1.86 | Mycobacterium tuberculosis | P9WFF7 | gene Rv2361c | - |
2.5.1.86 | Mycobacterium tuberculosis H37Rv | P9WFF7 | - |
- |
2.5.1.153 | Mycobacterium tuberculosis | P9WJ61 | bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9 | - |
2.5.1.153 | Mycobacterium tuberculosis ATCC 25618 | P9WJ61 | bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Mycobacterium tuberculosis | 7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the enzyme is a cis-prenyl transferase | Mycobacterium tuberculosis | 7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | 7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate | the enzyme is a cis-prenyl transferase | Mycobacterium tuberculosis H37Rv | 7 diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis | diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.86 | (2Z,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + trans,octacis-decaprenyl diphosphate | - |
? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | tuberculosinol + diphosphate | reaction of EC 3.1.7.8. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | Mycobacterium tuberculosis | tuberculosinol + diphosphate | - |
? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | tuberculosinol + diphosphate | reaction of EC 3.1.7.8. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | Mycobacterium tuberculosis ATCC 25618 | tuberculosinol + diphosphate | - |
? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | (13S)-isotuberculosinol + diphosphate | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis | (13S)-isotuberculosinol + diphosphate | reaction of EC 3.1.7.9. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | (13S)-isotuberculosinol + diphosphate | wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? | |
2.5.1.153 | tuberculosinyl diphosphate + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | (13S)-isotuberculosinol + diphosphate | reaction of EC 3.1.7.9. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.86 | dimer | - |
Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.86 | cis-decaprenyl diphosphate synthase | - |
Mycobacterium tuberculosis |
2.5.1.86 | decaprenyl diphosphate synthase | - |
Mycobacterium tuberculosis |
2.5.1.86 | DPPS | - |
Mycobacterium tuberculosis |
2.5.1.86 | Rv2361c | - |
Mycobacterium tuberculosis |
2.5.1.153 | Rv3378c | - |
Mycobacterium tuberculosis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.5.1.86 | 0.00041 | - |
pH and temperature not specified in the publication | Mycobacterium tuberculosis | BPH-640 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.86 | physiological function | the enzyme is involved in cell wall biosynthesis | Mycobacterium tuberculosis |