Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tang, Y.; Yang, S.; Yan, Q.; Zhou, P.; Cui, J.; Jiang, Z.
    Purification and characterization of a novel beta-1,3-1,4-glucanase (lichenase) from thermophilic Rhizomucor miehei with high specific activity and its gene sequence (2012), J. Agric. Food Chem., 60, 2354-2361.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 gene RmLic16A, DNA and amino acid sequence determination and analysis, sequence comparisons Rhizomucor miehei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 additional information
-
 additional information 2.0 mg/ml with barley beta-glucan and 1.4 mg/ml with lichenan at pH 5.5, 60°C Rhizomucor miehei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.73 extracellular
-
 Rhizomucor miehei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 31564
-
 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation Rhizomucor miehei
3.2.1.73 33700
-
 gel filtration Rhizomucor miehei
3.2.1.73 35400
-
 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Rhizomucor miehei H9BZ70 gene RmLic16A
-
3.2.1.73 Rhizomucor miehei CAU432 H9BZ70 gene RmLic16A
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.73 glycoprotein the enzyme contains two possible N-glycosylation sites at 72 (NKT) and 275 (NPT), respectively Rhizomucor miehei

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 native enzyme 6.8fold by ammonium sulfate fractionation and two different steps of anion exchange chromatography, to homogeneity Rhizomucor miehei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.73 additional information high-level extracellular enzyme production of 6230 U/mL on oat flour at 3% w/v as a carbon source at 50 °C Rhizomucor miehei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 28820 33480 purified native enzyme, substrate barley beta-glucan, pH 5.5, 60°C Rhizomucor miehei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 barley beta-glucan + H2O best substrate Rhizomucor miehei cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides, mainly trisaccharide and tetrasaccharide ?
3.2.1.73 barley beta-glucan + H2O best substrate Rhizomucor miehei CAU432 cellotriose + cellotetraose + cellobiose + D-glucose oligosaccharides, mainly trisaccharide and tetrasaccharide ?
3.2.1.73 lichenan + H2O 95.2% activity compared to barley beta-glucan Rhizomucor miehei cellotriose + ? oligosaccharides, mainly trisaccharide ?
3.2.1.73 lichenan + H2O 95.2% activity compared to barley beta-glucan Rhizomucor miehei CAU432 cellotriose + ? oligosaccharides, mainly trisaccharide ?
3.2.1.73 additional information thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview Rhizomucor miehei ?
-
 ?
3.2.1.73 additional information thin layer chromatography product analysis, overview. Poor activity with laminarin, no activity with birchwood xylan, carboxymethyl cellulose, pullulan, soluble starch, locust bean gum, cellulose, Avicel, beta-1,3-glucan, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside, substrate specificity, overview Rhizomucor miehei CAU432 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 monomer 1 * 35400, SDS-PAGE, 1 * 31564, sequence calculation Rhizomucor miehei
3.2.1.73 More peptide mapping of native enzyme Rhizomucor miehei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 beta-1,3-1,4-glucanase
-
 Rhizomucor miehei
3.2.1.73 endo-beta-1,3-1,4-glucanase
-
 Rhizomucor miehei
3.2.1.73 Lichenase
-
 Rhizomucor miehei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 60
-
-
 Rhizomucor miehei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.73 40 65 activity range, profile overview Rhizomucor miehei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 60
-
 purified native enzyme, no loss in activity at pH 5.5 for 30 min, half-life is 225 min Rhizomucor miehei
3.2.1.73 70
-
 purified native enzyme, pH 5.5, half-life is 101 min Rhizomucor miehei
3.2.1.73 75
-
 purified native enzyme, pH 5.5, half-life is 64 min Rhizomucor miehei
3.2.1.73 80
-
 purified native enzyme, pH 5.5, half-life is 53 min, 80% activity remaining after 30 min Rhizomucor miehei
3.2.1.73 85
-
 purified native enzyme, pH 5.5, half-life is 27 min, inactivation after 120 min Rhizomucor miehei
3.2.1.73 90
-
 purified native enzyme, pH 5.5, 60% activity remaining after 30 min Rhizomucor miehei
3.2.1.73 100
-
 purified native enzyme, pH 5.5, 40% activity remaining after 30 min Rhizomucor miehei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 5.5
-
-
 Rhizomucor miehei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 3.5 7.5 activity range, profile overview Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 5 7.5 purified native enzyme, retains 90% of its activity at 60°C for 30 min Rhizomucor miehei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.73 Rhizomucor miehei sequence calculation
-
 3.9

General Information

EC Number General Information Comment Organism
3.2.1.73 evolution the enzyme is a member of the glycosylhydrolase faamily GH16 Rhizomucor miehei