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Literature summary extracted from

  • Shao, H.; Xu, L.; Yan, Y.
    Biochemical characterization of a carboxylesterase from the archaeon Pyrobaculum sp. 1860 and a rational explanation of its substrate specificity and thermostability (2014), Int. J. Mol. Sci., 15, 16885-16910.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 functionally expressed in Escherichia coli as His-tagged protein Pyrobaculum sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 acetonitrile 15% v/v, 1.8fold activation. 30% v/v, 32% loss of activity Pyrobaculum sp.
3.1.1.1 Co2+ 1 mM, 5% inhibition. 10 mM, 27% inhibition Pyrobaculum sp.
3.1.1.1 Cu2+ 1 mM, 15% inhibition. 10 mM, 95% inhibition Pyrobaculum sp.
3.1.1.1 DMSO 30% v/v, 22% loss of activity Pyrobaculum sp.
3.1.1.1 ethanol 15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity Pyrobaculum sp.
3.1.1.1 Isopropanol 15% v/v, 1.4fold activation. 30% v/v, 21% loss of activity Pyrobaculum sp.
3.1.1.1 Mg2+ 1 mM, 5% loss of activity Pyrobaculum sp.
3.1.1.1 Mn2+ 1 mM; 10 mM, 12% loss of activity Pyrobaculum sp.
3.1.1.1 PMSF 1 mM, 28% loss of activity Pyrobaculum sp.
3.1.1.1 SDS 0.1% v/v, 17% loss of activity. 1% v/v, 68% loss of activity Pyrobaculum sp.
3.1.1.1 Tween-20 0.1% v/v, 16% loss of activity. 1% v/v, 33% loss of activity Pyrobaculum sp.
3.1.1.1 Zn2+ 1 mM, 8% inhibition. 10 mM, 49% inhibition Pyrobaculum sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.35
-
 4-nitrophenyl acetate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 1.27
-
 4-nitrophenyl butyrate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 2.64
-
 4-nitrophenyl octanoate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 4.99
-
 4-nitrophenyl decanoate pH 8.0, 80°C Pyrobaculum sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 acetone 15% or 30% v/v, 1.2fold activation Pyrobaculum sp.
3.1.1.1 acetonitrile 15% v/v, 1.8fold activation. 30% v/v, 32% loss of activity Pyrobaculum sp.
3.1.1.1 Ca2+ 10 mM, 10 mM, 1.1fold activation Pyrobaculum sp.
3.1.1.1 CTAB 0.1% v/v, 1.4fold activation. 1% v/v, 1.8fold activation Pyrobaculum sp.
3.1.1.1 ethanol 15% v/v, 1.7fold activation. 30% v/v, 18% loss of activity Pyrobaculum sp.
3.1.1.1 Isopropanol 15% v/v, 1.4fold activation. 30% v/v, 21% loss of activity Pyrobaculum sp.
3.1.1.1 K+ 10 mM, 1.2fold activation Pyrobaculum sp.
3.1.1.1 methanol 15% v/v, 1.4fold activation. 30% v/v, 1.7fold activation Pyrobaculum sp.
3.1.1.1 Mg2+ 10 mM, 1.2fold activation Pyrobaculum sp.
3.1.1.1 Na+ 10 mM, 1.1fold activation Pyrobaculum sp.
3.1.1.1 Triton X-100 0.1% v/v, 1.3fold activation. 1% v/v, 31% loss of activity Pyrobaculum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 21130
-
 calculated from sequence Pyrobaculum sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Pyrobaculum sp. G7VG08
-
-
3.1.1.1 Pyrobaculum sp. 1860 G7VG08
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
 Pyrobaculum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Pyrobaculum sp. 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O
-
 Pyrobaculum sp. 1860 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Pyrobaculum sp. 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O
-
 Pyrobaculum sp. 1860 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O
-
 Pyrobaculum sp. 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O
-
 Pyrobaculum sp. 1860 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Pyrobaculum sp. 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 Pyrobaculum sp. 1860 4-nitrophenol + octanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 P186_1588
-
 Pyrobaculum sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 80
-
-
 Pyrobaculum sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 40 90 40°C: about 50% of maximal activity, 90°C: 80% of maximal activity Pyrobaculum sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 additional information
-
 circular dichroism results indicated that rP186_1588 shows slight structure alteration from 60 to 90 °C. Comparative molecular simulations at different temperatures (27°C, 80°C, 100°C and 200°C) reveal that its thermostability is associated with its conformational rigidity Pyrobaculum sp.
3.1.1.1 90
-
 6 h, the enzyme retains 70% of relative activity Pyrobaculum sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 6.19
-
 4-nitrophenyl decanoate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 6.36
-
 4-nitrophenyl octanoate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 9.12
-
 4-nitrophenyl butyrate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 11.65
-
 4-nitrophenyl acetate pH 8.0, 80°C Pyrobaculum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 9
-
-
 Pyrobaculum sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 7 10 pH 7.0: about 50% of maxiomal activity, pH 10.0: about 60% of maximal activity Pyrobaculum sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Pyrobaculum sp. calculated
-
 6.3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 1.24
-
 4-nitrophenyl decanoate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 2.41
-
 4-nitrophenyl octanoate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 7.18
-
 4-nitrophenyl butyrate pH 8.0, 80°C Pyrobaculum sp.
3.1.1.1 33.29
-
 4-nitrophenyl acetate pH 8.0, 80°C Pyrobaculum sp.