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Literature summary extracted from

  • Balasubramanian, N.; Simoes, N.
    Bacillus pumilus S124A carboxymethyl cellulase; a thermo stable enzyme with a wide substrate spectrum utility (2014), Int. J. Biol. Macromol., 67, 132-139.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Cu2+ 1 mM, 40% residual activity Bacillus pumilus
3.2.1.4 EDTA 27% residual activity Bacillus pumilus
3.2.1.4 Fe2+ 1 mM, 37% residual activity Bacillus pumilus
3.2.1.4 Hg2+ 1 mM, 30% residual activity Bacillus pumilus
3.2.1.4 Phenylmethyl sulfonylfluoride 1 mM, 30% residual activity Bacillus pumilus
3.2.1.4 sodium dodecylsulfate 1%, 32% residual activity Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethyl cellulose Km value is 2.1 mg/ml, pH 6.0, 50°C Bacillus pumilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Bacillus pumilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 40000
-
 x * 40000, SDS-PAGE Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Bacillus pumilus
-
-
-
3.2.1.4 Bacillus pumilus S124A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus pumilus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Bacillus pumilus S124A ?
-
 ?
3.2.1.4 filter paper + H2O
-
 Bacillus pumilus ?
-
 ?
3.2.1.4 filter paper + H2O
-
 Bacillus pumilus S124A ?
-
 ?
3.2.1.4 additional information enzyme displays both beta-glucosidase, EC 3.2.1.21, and cellulase activities Bacillus pumilus ?
-
 ?
3.2.1.4 additional information enzyme displays both beta-glucosidase, EC 3.2.1.21, and cellulase activities Bacillus pumilus S124A ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 40000, SDS-PAGE Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
-
 Bacillus pumilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 8 more than 75% of maximum activity Bacillus pumilus