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Literature summary extracted from

  • Feliciano, P.R.; Gupta, S.; Dyszy, F.; Dias-Baruffi, M.; Costa-Filho, A.J.; Michels, P.A.; Nonato, M.C.
    Fumarate hydratase isoforms of Leishmania major: subcellular localization, structural and kinetic properties (2012), Int. J. Biol. Macromol., 51, 25-31.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.2 expressed in Escherichia coli as His-tagged fusion proteins Leishmania major

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 1.2
-
 S-malate pH 8.7, temperature not specified in the publication, aerobic condition, Vmax: 0.5 micromol/min/mg Leishmania major
4.2.1.2 1.3
-
 fumarate pH 8.7, temperature not specified in the publication, aerobic condition, Vmax: 1.8 micromol/min/mg Leishmania major
4.2.1.2 1.9
-
 fumarate pH 9, temperature not specified in the publication, aerobic condition, Vmax: 44.3 micromol/min/mg Leishmania major
4.2.1.2 2.3
-
 S-malate pH 8.7, temperature not specified in the publication, anaerobic condition, Vmax: 11.8 micromol/min/mg Leishmania major
4.2.1.2 2.5
-
 fumarate pH 8.7, temperature not specified in the publication, anaerobic condition, Vmax: 26.4 micromol/min/mg Leishmania major
4.2.1.2 5.7
-
 S-malate pH 9, temperature not specified in the publication, aerobic condition, Vmax: 22.7 micromol/min/mg Leishmania major
4.2.1.2 5.7
-
 fumarate pH 9, temperature not specified in the publication, anaerobic condition, Vmax: 186 micromol/min/mg Leishmania major
4.2.1.2 12.6
-
 S-malate pH 9, temperature not specified in the publication, anaerobic condition, Vmax: 138 micromol/min/mg Leishmania major

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.2 cytosol
-
 Leishmania major 5829
-
4.2.1.2 glycosome
-
 Leishmania major 20015
-
4.2.1.2 mitochondrion
-
 Leishmania major 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 61100
-
 calculated from cDNA Leishmania major
4.2.1.2 62600
-
 calculated from cDNA Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Leishmania major
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2 using Ni-NTA chromatography Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 S-malate
-
 Leishmania major fumarate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 LmFH-1
-
 Leishmania major
4.2.1.2 LmFH-2
-
 Leishmania major
4.2.1.2 LmjF24.0320
-
 Leishmania major
4.2.1.2 LmjF29.1960
-
 Leishmania major

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 56.3
-
 melting temperature Leishmania major
4.2.1.2 57.7
-
 melting temperature Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.2 0.55
-
 S-malate pH 8.7, temperature not specified in the publication, aerobic condition Leishmania major
4.2.1.2 1.9
-
 fumarate pH 8.7, temperature not specified in the publication, aerobic condition Leishmania major
4.2.1.2 12.9
-
 S-malate pH 8.7, temperature not specified in the publication, anaerobic condition Leishmania major
4.2.1.2 24.9
-
 S-malate pH 9, temperature not specified in the publication, aerobic condition Leishmania major
4.2.1.2 28.3
-
 fumarate pH 8.7, temperature not specified in the publication, anaerobic condition Leishmania major
4.2.1.2 48.6
-
 fumarate pH 9, temperature not specified in the publication, aerobic condition Leishmania major
4.2.1.2 151.4
-
 S-malate pH 9, temperature not specified in the publication, anaerobic condition Leishmania major
4.2.1.2 204.2
-
 fumarate pH 9, temperature not specified in the publication, anaerobic condition Leishmania major

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8.7
-
-
 Leishmania major
4.2.1.2 9
-
-
 Leishmania major