EC Number | General Stability | Organism |
---|---|---|
4.2.2.20 | the enzyme catalytic activity and intrinsic fluorescence intensity increase in the presence of cosolvents glycerol, sorbitol and trehalose whereas no considerable conformational changes are observed in far-UV CD spectra. In the presence of trehalose a significant increase in meltin temperature is observed. | Proteus vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.20 | 0.033 | - |
chondroitin 4-sulfate | presence of 1 M sorbitol, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 0.035 | - |
chondroitin 4-sulfate | presence of 1 M trehalose, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 0.039 | - |
chondroitin 4-sulfate | presence of 20% glycerol, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 0.044 | - |
chondroitin 4-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 0.055 | - |
chondroitin 6-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 0.094 | - |
dermatan sulfate | pH 6.8, 37°C | Proteus vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.20 | Proteus vulgaris | P59807 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Storage Stability | Organism |
---|---|---|
4.2.2.20 | -18°C, 3 days, complete loss of activity | Proteus vulgaris |
4.2.2.20 | 25°C, 17 days, complete loss of activity, in presence of 1 M trehalose 85% residual activity after 21 days | Proteus vulgaris |
4.2.2.20 | 37°C, 10 days, presence of 1 M trehalose, 20% residual activity | Proteus vulgaris |
4.2.2.20 | 4°C, 21 days, 80% residual activity | Proteus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.20 | chondroitin 4-sulfate | - |
Proteus vulgaris | ? | - |
? | |
4.2.2.20 | chondroitin 6-sulfate | - |
Proteus vulgaris | ? | - |
? | |
4.2.2.20 | dermatan sulfate | - |
Proteus vulgaris | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.20 | 37 | - |
- |
Proteus vulgaris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.20 | 47 | - |
melting temperature | Proteus vulgaris |
4.2.2.20 | 49 | - |
melting temperature, presence of 20% glycerol | Proteus vulgaris |
4.2.2.20 | 52 | - |
melting temperature, presence of 1 M sorbitol | Proteus vulgaris |
4.2.2.20 | 54 | - |
melting temperature, presence of 1 M trehalose | Proteus vulgaris |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.20 | 48.5 | - |
dermatan sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 69.7 | - |
chondroitin 4-sulfate | presence of 1 M sorbitol, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 75.8 | - |
chondroitin 4-sulfate | presence of 1 M trehalose, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 78.8 | - |
chondroitin 4-sulfate | presence of 20% glycerol, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 84.8 | - |
chondroitin 4-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 100 | - |
chondroitin 6-sulfate | pH 6.8, 37°C | Proteus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.20 | 6.8 | - |
- |
Proteus vulgaris |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.20 | 1517 | - |
dermatan sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 1800 | - |
chondroitin 6-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 1933 | - |
chondroitin 4-sulfate | pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 2017 | - |
chondroitin 4-sulfate | presence of 20% glycerol, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 2117 | - |
chondroitin 4-sulfate | presence of 1 M sorbitol, pH 6.8, 37°C | Proteus vulgaris | |
4.2.2.20 | 2167 | - |
chondroitin 4-sulfate | presence of 1 M trehalose, pH 6.8, 37°C | Proteus vulgaris |