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Literature summary extracted from

  • Nazari-Robati, M.; Khajeh, K.; Aminian, M.; Fathi-Roudsari, M.; Golestani, A.
    Co-solvent mediated thermal stabilization of chondroitinase ABC I form Proteus vulgaris (2012), Int. J. Biol. Macromol., 50, 487-492.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.2.20 the enzyme catalytic activity and intrinsic fluorescence intensity increase in the presence of cosolvents glycerol, sorbitol and trehalose whereas no considerable conformational changes are observed in far-UV CD spectra. In the presence of trehalose a significant increase in meltin temperature is observed. Proteus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.20 0.033
-
chondroitin 4-sulfate presence of 1 M sorbitol, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 0.035
-
chondroitin 4-sulfate presence of 1 M trehalose, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 0.039
-
chondroitin 4-sulfate presence of 20% glycerol, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 0.044
-
chondroitin 4-sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 0.055
-
chondroitin 6-sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 0.094
-
dermatan sulfate pH 6.8, 37°C Proteus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.20 Proteus vulgaris P59807
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
4.2.2.20 -18°C, 3 days, complete loss of activity Proteus vulgaris
4.2.2.20 25°C, 17 days, complete loss of activity, in presence of 1 M trehalose 85% residual activity after 21 days Proteus vulgaris
4.2.2.20 37°C, 10 days, presence of 1 M trehalose, 20% residual activity Proteus vulgaris
4.2.2.20 4°C, 21 days, 80% residual activity Proteus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.20 chondroitin 4-sulfate
-
Proteus vulgaris ?
-
?
4.2.2.20 chondroitin 6-sulfate
-
Proteus vulgaris ?
-
?
4.2.2.20 dermatan sulfate
-
Proteus vulgaris ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.20 37
-
-
Proteus vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.20 47
-
melting temperature Proteus vulgaris
4.2.2.20 49
-
melting temperature, presence of 20% glycerol Proteus vulgaris
4.2.2.20 52
-
melting temperature, presence of 1 M sorbitol Proteus vulgaris
4.2.2.20 54
-
melting temperature, presence of 1 M trehalose Proteus vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.20 48.5
-
dermatan sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 69.7
-
chondroitin 4-sulfate presence of 1 M sorbitol, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 75.8
-
chondroitin 4-sulfate presence of 1 M trehalose, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 78.8
-
chondroitin 4-sulfate presence of 20% glycerol, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 84.8
-
chondroitin 4-sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 100
-
chondroitin 6-sulfate pH 6.8, 37°C Proteus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.20 6.8
-
-
Proteus vulgaris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.2.20 1517
-
dermatan sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 1800
-
chondroitin 6-sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 1933
-
chondroitin 4-sulfate pH 6.8, 37°C Proteus vulgaris
4.2.2.20 2017
-
chondroitin 4-sulfate presence of 20% glycerol, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 2117
-
chondroitin 4-sulfate presence of 1 M sorbitol, pH 6.8, 37°C Proteus vulgaris
4.2.2.20 2167
-
chondroitin 4-sulfate presence of 1 M trehalose, pH 6.8, 37°C Proteus vulgaris