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Literature summary extracted from

  • Ben Bacha, A.; Daihan, S.K.; Moubayed, N.M.; Mejdoub, H.
    Purification and characterization of a phospholipase A2-IIA from common stingray (Dasyatis pastinaca) intestine (2013), Indian J. Biochem. Biophys., 50, 186-195.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 sodium deoxycholate or sodium taurodeoxycholate, required, optimum concentration 6 mM Dasyatis pastinaca
3.1.1.4 sodium taurodeoxycholate or sodium deoxycholate, required, optimum concentration 4 mM Dasyatis pastinaca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 EDTA 10 mM, complete inhibition Dasyatis pastinaca
3.1.1.4 EGTA 10 mM, complete inhibition Dasyatis pastinaca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ absolutely required, optimum concentration 8 mM Dasyatis pastinaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.4 14000
-
 PAGE Dasyatis pastinaca

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.4 2-propanol 50%, 97% of initial activity Dasyatis pastinaca
3.1.1.4 Acetone 50%, no changes in activity Dasyatis pastinaca
3.1.1.4 acetonitrile 50%, 15% increase in activity Dasyatis pastinaca
3.1.1.4 Ethanol 50%, 23% increase in activity Dasyatis pastinaca
3.1.1.4 Methanol 50%, no changes in activity Dasyatis pastinaca

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Dasyatis pastinaca
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.4 glycoprotein small amount of N-glycosylation Dasyatis pastinaca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.4
-
 Dasyatis pastinaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 intestine
-
 Dasyatis pastinaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.4 290
-
 pH 9.5, 45°C, presence of sodium deoxycholate and CaCl2 Dasyatis pastinaca

Storage Stability

EC Number Storage Stability Organism
3.1.1.4 -20°C, 120 days, 95% residual activity Dasyatis pastinaca
3.1.1.4 4°C, 120 days, 85% residual activity Dasyatis pastinaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1-stearoyl-2-oleoyl-sn-glycerol-3-phosphocholine + H2O
-
 Dasyatis pastinaca 1-stearoyl-sn-glycero-3-phosphocholine + oleic acid hydrolysis occurs only in sn2 position of phospholipids ?
3.1.1.4 egg yolk + H2O
-
 Dasyatis pastinaca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 monomer 1 * 14000, SDS-PAGE Dasyatis pastinaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 45
-
-
 Dasyatis pastinaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.4 70
-
 20 min, 80% residual activity Dasyatis pastinaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 9.5
-
-
 Dasyatis pastinaca

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.4 2
-
 maintains more than 70% of activity after incubation at pH 2 Dasyatis pastinaca