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Literature summary extracted from
- Heparosan-glucuronate 5-epimerase: molecular cloning and characterization of a novel enzyme (2015), Glycobiology, 25, 735-744.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.3.36 | expressed in insect cells | Lissachatina fulica |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.3.36 | CaCl2 | - |
Lissachatina fulica |  |
| 5.1.3.36 | CHAPS | 0.1%, completely abolishes activity | Lissachatina fulica | |
| 5.1.3.36 | MgCl2 | - |
Lissachatina fulica | |
| 5.1.3.36 | MnCl2 | - |
Lissachatina fulica | |
| 5.1.3.36 | NaCl | - |
Lissachatina fulica | |
| 5.1.3.36 | sodium deoxycholate | - |
Lissachatina fulica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.36 | 75000 | - |
x * 75000, SDS-PAGE | Lissachatina fulica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.36 | [heparosan]-D-glucuronate | Lissachatina fulica | it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate | [heparosan]-L-iduronate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.36 | Lissachatina fulica | A0A0C6EX43 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.3.36 | - |
Lissachatina fulica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.36 | [heparosan]-D-glucuronate | it is suggested that the enzyme participates in the biosynthetic pathway of acharan sulfate | Lissachatina fulica | [heparosan]-L-iduronate | - |
? | |
| 5.1.3.36 | [heparosan]-D-glucuronate | no activity with [heparosan]-N-sulfate D-glucuronate. At equilibrium of the epimerization, the proportion of IdoA in the reaction product reaches up to 30% of total hexuronic acid | Lissachatina fulica | [heparosan]-L-iduronate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.3.36 | ? | x * 75000, SDS-PAGE | Lissachatina fulica |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.3.36 | HG-5epi | - |
Lissachatina fulica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.36 | 30 | - |
assay at | Lissachatina fulica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.36 | 35 | - |
unstable above | Lissachatina fulica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.36 | 6 | - |
- |
Lissachatina fulica |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.3.36 | 5.5 | 7 | pH 5.0: about 50% of maximal activity, pH 7.0: about 40% of maximal activity | Lissachatina fulica |
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Release 2023.1 (January 2023)
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