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Literature summary extracted from

  • But, S.Y.; Khmelenina, V.N.; Reshetnikov, A.S.; Trotsenko, Y.A.
    Bifunctional sucrose phosphate synthase/phosphatase is involved in the sucrose biosynthesis by Methylobacillus flagellatus KT (2013), FEMS Microbiol. Lett., 347, 43-51.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 ATP
-
 Methylobacillus flagellatus
2.4.1.14 D-fructose 1,6-bisphosphate
-
 Methylobacillus flagellatus
3.1.3.24 ATP 148% activity at 5 mM Methylobacillus flagellatus
3.1.3.24 D-fructose 1,6-bisphosphate 124% activity at 5 mM Methylobacillus flagellatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.14 gene sps, sequence comparisons, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli Methylobacillus flagellatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 AMP
-
 Methylobacillus flagellatus
2.4.1.14 D-Fructose 1-phosphate slight inhibition Methylobacillus flagellatus
2.4.1.14 D-glucose 6-phosphate
-
 Methylobacillus flagellatus
2.4.1.14 D-ribose 5-phosphate
-
 Methylobacillus flagellatus
2.4.1.14 additional information poor effect by fructose and sucrose Methylobacillus flagellatus
2.4.1.14 phosphate slight inhibition Methylobacillus flagellatus
3.1.3.24 AMP 68% residual activity at 5 mM Methylobacillus flagellatus
3.1.3.24 D-Fructose 1-phosphate 62% residual activity at 5 mM Methylobacillus flagellatus
3.1.3.24 D-glucose 6-phosphate 67% residual activity at 5 mM Methylobacillus flagellatus
3.1.3.24 D-ribose 5-phosphate 9% residual activity at 5 mM Methylobacillus flagellatus
3.1.3.24 additional information not inhibited by D-glucose 1-phosphate, D-fructose, and sucrose Methylobacillus flagellatus
3.1.3.24 phosphate complete inhibition at 5 mM Methylobacillus flagellatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 0.19
-
 sucrose 6F-phosphate pH 6.5, 45°C, recombinant His6-tagged enzyme Methylobacillus flagellatus
2.4.1.14 1.2
-
 D-fructose 6-phosphate pH 6.5, 45°C, recombinant His6-tagged enzyme Methylobacillus flagellatus
2.4.1.14 1.3
-
 UDP-glucose pH 6.5, 45°C, recombinant His6-tagged enzyme, in presence of fructose 1,6-bisphosphate Methylobacillus flagellatus
3.1.3.24 0.19
-
 sucrose 6-phosphate at pH 6.5 and 45°C Methylobacillus flagellatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.14 Mg2+ dependent on, optimal at 17.5 mM Methylobacillus flagellatus
2.4.1.14 NaCl activates, best at 2% Methylobacillus flagellatus
3.1.3.24 Mg2+ dependent on, the optimal ion concentration is 17.5 mM Methylobacillus flagellatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 84132
-
 4 * 84132, sequence calculation Methylobacillus flagellatus
2.4.1.14 336000
-
 gel filtration Methylobacillus flagellatus
3.1.3.24 84000
-
 4 * 84000, SDS-PAGE Methylobacillus flagellatus
3.1.3.24 336000
-
 gel filtration Methylobacillus flagellatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 additional information Methylobacillus flagellatus a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) ?
-
 ?
2.4.1.14 UDP-glucose + D-fructose 6-phosphate Methylobacillus flagellatus
-
 UDP + sucrose 6F-phosphate
-
 r
3.1.3.24 sucrose 6-phosphate + H2O Methylobacillus flagellatus
-
 sucrose + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Methylobacillus flagellatus Q1GY13
-
-
3.1.3.24 Methylobacillus flagellatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Methylobacillus flagellatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 additional information a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) Methylobacillus flagellatus ?
-
 ?
2.4.1.14 UDP + sucrose 6F-phosphate
-
 Methylobacillus flagellatus UDP-glucose + D-fructose 6-phosphate
-
 r
2.4.1.14 UDP-glucose + D-fructose 6-phosphate
-
 Methylobacillus flagellatus UDP + sucrose 6F-phosphate
-
 r
3.1.3.24 sucrose 6-phosphate + H2O
-
 Methylobacillus flagellatus sucrose + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.14 homotetramer 4 * 84132, sequence calculation Methylobacillus flagellatus
3.1.3.24 homotetramer 4 * 84000, SDS-PAGE Methylobacillus flagellatus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.14 SPS
-
 Methylobacillus flagellatus
3.1.3.24 SPS/SPP bifunctional enzyme with sucrose phosphate synthase/phosphatase activities Methylobacillus flagellatus
3.1.3.24 sucrose 6-phosphate phosphohydrolase
-
 Methylobacillus flagellatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.14 45
-
-
 Methylobacillus flagellatus
3.1.3.24 45
-
-
 Methylobacillus flagellatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 6.5
-
-
 Methylobacillus flagellatus
3.1.3.24 6.5
-
-
 Methylobacillus flagellatus