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Literature summary extracted from

  • Vandamme, A.M.; Michaux, C.; Mayard, A.; Housen, I.
    Asparagine 42 of the conserved endo-inulinase INU2 motif WMNDPN from Aspergillus ficuum plays a role in activity specificity (2013), FEBS Open Bio, 3, 467-472.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.7 D298A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 59.7% of wild-typ activity Aspergillus ficuum
3.2.1.7 E43D mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 5.4% of wild-typ activity Aspergillus ficuum
3.2.1.7 F99A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 6.1% of wild-typ activity Aspergillus ficuum
3.2.1.7 I70A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 38.9% of wild-typ activity Aspergillus ficuum
3.2.1.7 M41A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 56.2% of wild-typ activity Aspergillus ficuum
3.2.1.7 N265A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 72.9% of wild-typ activity Aspergillus ficuum
3.2.1.7 N42G mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 7.1% of wild-typ activity Aspergillus ficuum
3.2.1.7 P62G mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 0.2% of wild-typ activity Aspergillus ficuum
3.2.1.7 Q59A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 2.2% of wild-typ activity Aspergillus ficuum
3.2.1.7 R175A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 0.1% of wild-typ activity Aspergillus ficuum
3.2.1.7 R295A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 84.6% of wild-typ activity Aspergillus ficuum
3.2.1.7 W67A mutation within the enlarged cavity formed by the conserved motif W-M(I)-N-D(E)-P-N-G, the so-called loop 1 and the loop 4. 2.4% of wild-typ activity Aspergillus ficuum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus ficuum O94220
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Aspergillus ficuum inulotetraose + inulotriose enzyme produces equal amounts of inulotetraose and inulotriose ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.7 Inu2
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 Aspergillus ficuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 50
-
-
 Aspergillus ficuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 5
-
-
 Aspergillus ficuum