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Literature summary extracted from

  • Tannous, E.; Yokoyama, K.; You, D.J.; Koga, Y.; Kanaya, S.
    A dual role of divalent metal ions in catalysis and folding of RNase H1 from extreme halophilic archaeon Halobacterium sp. NRC-1 (2012), FEBS Open Bio, 2, 345-352.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.13.2 expressed in Escherichia coli BL21-CodonPlus(DE3) cells Halobacterium sp.
3.1.26.4 expressed in Escherichia coli BL21-CodonPlus(DE3) cells Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.13.2 Mg2+ the optimum concentration of Mg2+ for activity in the presence of 3 M NaCl is 20 mM Halobacterium sp.
3.1.13.2 Mn2+ the optimum concentration of Mn2+ for activity in the presence of 3 M NaCl is 1 mM Halobacterium sp.
3.1.13.2 additional information the enzyme requires either salt or divalent metal ions for folding and is incompletely folded in the absence of both of them Halobacterium sp.
3.1.26.4 additional information the enzyme requires either salt or divalent metal ions for folding. The enzyme exhibits activity in the presence of divalent metal ions regardless of the presence or absence of 3 M NaCl. However, higher concentrations of divalent metal ions are required for activity in the absence of salt to facilitate folding Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.4 24000
-
 x * 24000, SDS-PAGE Halobacterium salinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.13.2 12 bp RNA/DNA hybrid + H2O Halobacterium sp. 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA ?
-
 ?
3.1.13.2 12 bp RNA/DNA hybrid + H2O Halobacterium sp. NRC-1 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA ?
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O Halobacterium salinarum
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O Halobacterium salinarum NRC 1
-
 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.13.2 Halobacterium sp.
-
-
-
3.1.13.2 Halobacterium sp. NRC-1
-
-
-
3.1.26.4 Halobacterium salinarum Q9HSF6
-
-
3.1.26.4 Halobacterium salinarum NRC 1 Q9HSF6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.13.2 HiTrap Q column chromatography, Mono Q column chromatography, and Superdex 200pg gel filtration Halobacterium sp.
3.1.26.4 HiTrap Q column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.13.2 12 bp RNA/DNA hybrid + H2O 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA Halobacterium sp. ?
-
 ?
3.1.13.2 12 bp RNA/DNA hybrid + H2O 5'-(6-carboxyfluorescein)-labeled 12 base RNA (5'-cggagaugacgg-3') is hybridized with with a 1.5 M equivalent of the complementary DNA Halobacterium sp. NRC-1 ?
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O
-
 Halobacterium salinarum ribonucleotide 5'-phosphomonoester + ?
-
 ?
3.1.26.4 RNA-DNA hybrid + H2O
-
 Halobacterium salinarum NRC 1 ribonucleotide 5'-phosphomonoester + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.4 ? x * 24000, SDS-PAGE Halobacterium salinarum

Synonyms

EC Number Synonyms Comment Organism
3.1.13.2 RNase H1
-
 Halobacterium sp.
3.1.13.2 RNH1
-
 Halobacterium sp.
3.1.26.4 RNase H1
-
 Halobacterium salinarum
3.1.26.4 RNH1
-
 Halobacterium salinarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.26.4 46.2 76.5 the melting temperature in the presence of 300 mM MgCl2, 20 mM MnCl2, 3 M NaCl, or 300 mM MgCl2 plus 3 M NaCl is 46.2°C, 50°C, 66.9°C, and 76.5°C, respectively Halobacterium salinarum