EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.4 | expressed in Escherichia coli MIC2067(DE3) cells | Geobacillus stearothermophilus |
3.1.26.4 | expressed in Escherichia coli MIC2067(DE3) cells | Thermotoga maritima |
EC Number | General Stability | Organism |
---|---|---|
3.1.26.4 | the C-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain | Thermotoga maritima |
3.1.26.4 | the N-terminal extension of the enzyme functions as a substrate-binding domain and stabilizes the RNase H domain | Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.26.4 | Mg2+ | the optimum concentration is 1 mM | Thermotoga maritima | |
3.1.26.4 | Mg2+ | the optimum concentration is 10 mM | Geobacillus stearothermophilus | |
3.1.26.4 | Mn2+ | the optimum concentration is 1 mM | Thermotoga maritima | |
3.1.26.4 | Mn2+ | the optimum concentration is 10 mM | Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.26.4 | 26630 | - |
1 * 26630, SDS-PAGE | Thermotoga maritima |
3.1.26.4 | 27000 | - |
gel filtration | Thermotoga maritima |
3.1.26.4 | 28892 | - |
1 * 28892, SDS-PAGE | Geobacillus stearothermophilus |
3.1.26.4 | 32000 | - |
gel filtration | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.4 | 12 base pair RNA-DNA hybrid + H2O | Geobacillus stearothermophilus | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? | |
3.1.26.4 | 12 base pair RNA-DNA hybrid + H2O | Thermotoga maritima | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? | |
3.1.26.4 | 12 base pair RNA-DNA hybrid + H2O | Thermotoga maritima DSM 3109 | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.4 | Geobacillus stearothermophilus | - |
- |
- |
3.1.26.4 | Thermotoga maritima | Q9X017 | - |
- |
3.1.26.4 | Thermotoga maritima DSM 3109 | Q9X017 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.4 | HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration | Geobacillus stearothermophilus |
3.1.26.4 | HiTrap Q column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.4 | 12 base pair RNA-DNA hybrid + H2O | - |
Geobacillus stearothermophilus | ribonucleotide 5'-phosphomonoester + ? | - |
? | |
3.1.26.4 | 12 base pair RNA-DNA hybrid + H2O | - |
Thermotoga maritima | ribonucleotide 5'-phosphomonoester + ? | - |
? | |
3.1.26.4 | 12 base pair RNA-DNA hybrid + H2O | - |
Thermotoga maritima DSM 3109 | ribonucleotide 5'-phosphomonoester + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.4 | monomer | 1 * 26630, SDS-PAGE | Thermotoga maritima |
3.1.26.4 | monomer | 1 * 28892, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.4 | RNase H2 | - |
Geobacillus stearothermophilus |
3.1.26.4 | RNase H2 | - |
Thermotoga maritima |
3.1.26.4 | RNH2 | - |
Geobacillus stearothermophilus |
3.1.26.4 | RNH2 | - |
Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.4 | 53.1 | - |
melting temperature | Geobacillus stearothermophilus |
3.1.26.4 | 66.1 | - |
melting temperature | Thermotoga maritima |