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Literature summary extracted from

  • Meena, S.; Gothwal, R.K.; Krishna Mohan, M.; Ghosh, P.
    Production and purification of a hyperthermostable chitinase from Brevibacillus formosus BISR-1 isolated from the Great Indian Desert soils (2014), Extremophiles, 18, 451-462.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular the enzyme is secreted Brevibacillus formosus
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 37600
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 x * 37600 Brevibacillus formosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Brevibacillus formosus
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 ?
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 ?
3.2.1.14 chitin + H2O Brevibacillus formosus BISR-1
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 ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Brevibacillus formosus
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 isolated from the Great Indian Desert soils
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3.2.1.14 Brevibacillus formosus BISR-1
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 isolated from the Great Indian Desert soils
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native extracellular enzyme 6.1fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, and adsorption chromatography Brevibacillus formosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 additional information high level enzyme expression in strain BISR-1, optimized conditions with medium containing chitin 2.0 g/l, malt extract 1.5 g/l, glycerol 1.0 g/l, ammonium nitrate 0.3%, T-20 0.1%, pH 7.0, 37°C, optimization process, detailed overview. Temperature growth limit is 50°C, sharp decrase in enzyme activity at 45°C. Effects of surfactants on enzyme production, overview Brevibacillus formosus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
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 203 U/ml, pH 7.0, 37°C, optimized enzyme production conditions Brevibacillus formosus
3.2.1.14 94.21
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 purified enzyme, pH 7.0, 37°C Brevibacillus formosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
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 Brevibacillus formosus ?
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 ?
3.2.1.14 chitin + H2O
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 Brevibacillus formosus BISR-1 ?
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 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 37600 Brevibacillus formosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 55
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-
 Brevibacillus formosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 35 100 activity range Brevibacillus formosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 80
-
 purified native enzyme, 60% activity remaining after 72 h Brevibacillus formosus
3.2.1.14 90
-
 purified native enzyme, half-life is 72 h Brevibacillus formosus
3.2.1.14 100
-
 purified native enzyme, half-life is 5 h Brevibacillus formosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
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-
 Brevibacillus formosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 10 activity range Brevibacillus formosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 4 10 the purified enzyme retains over 65% residual activity in this range Brevibacillus formosus