BRENDA - Enzyme Database show

Plasmodium falciparum Sir2: an unusual sirtuin with dual histone deacetylase and ADP-ribosyltransferase activity

Merrick, C.J.; Duraisingh, M.T.; Eukaryot. Cell 6, 2081-291 (2007)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.B41
additional information
the enzyme is unaffected by the activators isonicotinamide and resveratrol
Plasmodium falciparum
Application
EC Number
Application
Commentary
Organism
2.3.1.B41
medicine
PfSir2A inhibitors may have therapeutic value in malaria treatment
Plasmodium falciparum
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.B41
expressed in Escherichia coli with a C-terminal His6 tag and an N-terminal T7 tag
Plasmodium falciparum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.B41
additional information
splitomicin and sirtinol fail to inhibit PfSir2
Plasmodium falciparum
2.3.1.B41
nicotinamide
inhibits the deacetylation of native histones much more effectively than deacetylation of a synthetic substrate
Plasmodium falciparum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.B41
30000
-
x * 30000, SDS-PAGE
Plasmodium falciparum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.B41
Plasmodium falciparum
Q8IE47
-
-
2.3.1.B41
Plasmodium falciparum isolate 3D7
Q8IE47
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.B41
-
Plasmodium falciparum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.B41
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
2.3.1.B41
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
2.3.1.B41
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
2.3.1.B41
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.B41
?
x * 30000, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.B41
37
-
assay at
Plasmodium falciparum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.B41
additional information
the enzyme is unaffected by the activators isonicotinamide and resveratrol
Plasmodium falciparum
Application (protein specific)
EC Number
Application
Commentary
Organism
2.3.1.B41
medicine
PfSir2A inhibitors may have therapeutic value in malaria treatment
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.B41
expressed in Escherichia coli with a C-terminal His6 tag and an N-terminal T7 tag
Plasmodium falciparum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.B41
additional information
splitomicin and sirtinol fail to inhibit PfSir2
Plasmodium falciparum
2.3.1.B41
nicotinamide
inhibits the deacetylation of native histones much more effectively than deacetylation of a synthetic substrate
Plasmodium falciparum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.B41
30000
-
x * 30000, SDS-PAGE
Plasmodium falciparum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.B41
-
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.B41
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
2.3.1.B41
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
2.3.1.B41
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
2.3.1.B41
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.B41
?
x * 30000, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.B41
37
-
assay at
Plasmodium falciparum