EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.1 | expression in Escherichia coli | Ferroplasma acidiphilum |
3.2.1.20 | expression in Escherichia coli | Ferroplasma acidiphilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.45 | - |
4-nitrophenyl propionate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 0.47 | - |
4-nitrophenyl acetate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 0.6 | - |
4-nitrophenyl butyrate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 2.2 | - |
Triacetin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 3.5 | - |
tripropionin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 3.9 | - |
4-nitrophenyl hexanoate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 7.4 | - |
tributyrin | pH 2.0, 50°C | Ferroplasma acidiphilum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.1 | membrane | - |
Ferroplasma acidiphilum | 16020 | - |
3.2.1.20 | intracellular | - |
Ferroplasma acidiphilum | 5622 | - |
3.2.1.20 | membrane | - |
Ferroplasma acidiphilum | 16020 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 34734 | - |
1 * 34734, calculated from sequence | Ferroplasma acidiphilum |
3.1.1.1 | 35000 | - |
non-denasturing PAGE | Ferroplasma acidiphilum |
3.1.1.1 | 36000 | - |
1 * 36000, SDS-PAGE | Ferroplasma acidiphilum |
3.2.1.20 | 28316 | - |
1 * 28316, calculated from sequence | Ferroplasma acidiphilum |
3.2.1.20 | 40505 | - |
1 * 40505, calculated from sequence | Ferroplasma acidiphilum |
3.2.1.20 | 42000 | - |
1 * 42000, SDS-PAGE | Ferroplasma acidiphilum |
3.2.1.20 | 56000 | - |
non-denasturing PAGE | Ferroplasma acidiphilum |
3.2.1.20 | 57000 | - |
non-denaturing PAGE | Ferroplasma acidiphilum |
3.2.1.20 | 57300 | - |
1 * 57300, calculated from sequence | Ferroplasma acidiphilum |
3.2.1.20 | 80000 | - |
non-denasturing PAGE | Ferroplasma acidiphilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.1 | Ferroplasma acidiphilum | Q2PCE5 | - |
- |
3.2.1.20 | Ferroplasma acidiphilum | Q2PCE2 | - |
- |
3.2.1.20 | Ferroplasma acidiphilum | Q2PCE3 | - |
- |
3.2.1.20 | Ferroplasma acidiphilum | Q5K3Q3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.1 | purified from both Escherichia coli and Ferroplasma acidiphilum | Ferroplasma acidiphilum |
3.2.1.20 | purified from both Escherichia coli and Ferroplasma acidiphilum | Ferroplasma acidiphilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + acetate | - |
? | |
3.1.1.1 | 4-nitrophenyl butyrate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + butyrate | - |
? | |
3.1.1.1 | 4-nitrophenyl hexanoate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + hexanoate | - |
? | |
3.1.1.1 | 4-nitrophenyl propionate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + propionate | - |
? | |
3.1.1.1 | additional information | no activity with triolein | Ferroplasma acidiphilum | ? | - |
? | |
3.1.1.1 | triacetin + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
3.1.1.1 | tributyrin + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
3.1.1.1 | tripropionin + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
3.2.1.20 | maltose + H2O | - |
Ferroplasma acidiphilum | 2 D-glucose | - |
? | |
3.2.1.20 | maltotetraose + H2O | very low activity | Ferroplasma acidiphilum | ? | - |
? | |
3.2.1.20 | maltotriose + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
3.2.1.20 | sucrose + H2O | - |
Ferroplasma acidiphilum | D-glucose + D-fructose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.1 | monomer | 1 * 36000, SDS-PAGE | Ferroplasma acidiphilum |
3.1.1.1 | monomer | 1 * 34734, calculated from sequence | Ferroplasma acidiphilum |
3.2.1.20 | ? | 1 * 42000, SDS-PAGE | Ferroplasma acidiphilum |
3.2.1.20 | ? | 1 * 28316, calculated from sequence | Ferroplasma acidiphilum |
3.2.1.20 | ? | 1 * 40505, calculated from sequence | Ferroplasma acidiphilum |
3.2.1.20 | ? | 1 *28000, SDS-PAGE | Ferroplasma acidiphilum |
3.2.1.20 | monomer | 1 * 57000, SDS-PAGE | Ferroplasma acidiphilum |
3.2.1.20 | monomer | 1 * 57300, calculated from sequence | Ferroplasma acidiphilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.1 | EStFa | - |
Ferroplasma acidiphilum |
3.2.1.20 | alphaGluFa | - |
Ferroplasma acidiphilum |
3.2.1.20 | GlyFa1 | - |
Ferroplasma acidiphilum |
3.2.1.20 | GlyFa2 | - |
Ferroplasma acidiphilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 50 | - |
pH 1.0-2.5, hydrolysis of 4-nitrophenol propionate | Ferroplasma acidiphilum |
3.2.1.20 | 60 | - |
- |
Ferroplasma acidiphilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 50 | - |
pH 1.7-2.0, half-life: 48 min | Ferroplasma acidiphilum |
3.2.1.20 | 60 | - |
pH 1.7-2.5, half-life: 23 min | Ferroplasma acidiphilum |
3.2.1.20 | 60 | - |
pH 2.5-3.5, half-life: 34 min | Ferroplasma acidiphilum |
3.2.1.20 | 60 | - |
pH 3.5-4.0, half-life: 12 min | Ferroplasma acidiphilum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 2 | - |
4-nitrophenyl hexanoate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 10 | - |
4-nitrophenyl butyrate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 23 | - |
tributyrin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 25 | - |
4-nitrophenyl acetate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 29 | - |
4-nitrophenyl propionate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 44 | - |
tripropionin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 47 | - |
Triacetin | pH 2.0, 50°C | Ferroplasma acidiphilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 1.5 | - |
- |
Ferroplasma acidiphilum |
3.2.1.20 | 2 | - |
- |
Ferroplasma acidiphilum |
3.2.1.20 | 2.5 | 3 | - |
Ferroplasma acidiphilum |
3.2.1.20 | 3.5 | 4 | - |
Ferroplasma acidiphilum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.1 | 1 | 3 | pH 1.0: about 70% of maximal activity, pH 3.0: about 65% of maximal activity | Ferroplasma acidiphilum |
3.2.1.20 | 1 | 4 | pH 1.0: about 45% of maximal activity, pH 4.0: about 55% of maximal activity | Ferroplasma acidiphilum |
3.2.1.20 | 1.5 | 5 | pH 1.5: about 70% of maximal activity, pH 5.0: about 60% of maximal activity | Ferroplasma acidiphilum |
3.2.1.20 | 2.5 | 5.5 | pH 2.5: about 40% of maximal activity, pH 5.5: about 40% of maximal activity | Ferroplasma acidiphilum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.1 | Ferroplasma acidiphilum | calculated from sequence | - |
5.91 |
3.2.1.20 | Ferroplasma acidiphilum | calculated from sequence | - |
6.42 |
3.2.1.20 | Ferroplasma acidiphilum | calculated from sequence | - |
9.53 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.1 | 0.6 | - |
4-nitrophenyl hexanoate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 3.1 | - |
tributyrin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 12.7 | - |
tripropionin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 16.7 | - |
4-nitrophenyl butyrate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 21.3 | - |
Triacetin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 53 | - |
4-nitrophenyl acetate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.1.1.1 | 64.2 | - |
4-nitrophenyl propionate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
3.2.1.20 | 0.1 | - |
maltotetraose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 0.3 | - |
maltotetraose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 0.6 | - |
maltotetraose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 18.8 | - |
maltotriose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 28 | - |
maltotriose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 37 | - |
maltotriose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 73 | - |
maltose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 101 | - |
maltose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 142 | - |
sucrose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 197 | - |
maltose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum | |
3.2.1.20 | 293 | - |
sucrose | 60°C, pH not specified in the publication | Ferroplasma acidiphilum |