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Literature summary extracted from

  • Cerda-Maira, F.A.; McAllister, F.; Bode, N.J.; Burns, K.E., Gygi, S.P.; Darwin, K.H.
    Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli (2011), EMBO Rep., 12, 863-870.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.19 reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.19 Mycobacterium tuberculosis P9WNU7
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6.3.1.19 Mycobacterium tuberculosis ATCC 25618 P9WNU7
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [adenylate kinase-His6]-L-lysine adenylate kinase from Escherichia coli is pupylated Mycobacterium tuberculosis ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[adenylate kinase-His6]-L-lysine
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 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [adenylate kinase-His6]-L-lysine adenylate kinase from Escherichia coli is pupylated Mycobacterium tuberculosis ATCC 25618 ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[adenylate kinase-His6]-L-lysine
-
 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine Lys173 is the predominant modified residue in malonyl Co-A acyl carrier protein transacylase (FabD) purified from Escherichia coli. Lys122 and Lys181 are also identified as pupylation targets. At least three lysines in Mtb FabD can be pupylated in Escherichia coli. It also seems that only one residue per FabD polypeptide is pupylated at one time Mycobacterium tuberculosis ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine
-
 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [malonyl Co-A acyl carrier protein transacylase]-L-lysine Lys173 is the predominant modified residue in malonyl Co-A acyl carrier protein transacylase (FabD) purified from Escherichia coli. Lys122 and Lys181 are also identified as pupylation targets. At least three lysines in Mtb FabD can be pupylated in Escherichia coli. It also seems that only one residue per FabD polypeptide is pupylated at one time Mycobacterium tuberculosis ATCC 25618 ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[malonyl Co-A acyl carrier protein transacylase]-L-lysine
-
 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [myo-inositol-1-phosphate synthetase]-L-lysine expression of Mycobacterium tuberculosis ino1, pafA and pupGlu, results in pupylated myo-inositol-1-phosphate synthetase (Ino1) in Escherichia coli Mycobacterium tuberculosis ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[myo-inositol-1-phosphate synthetase]-L-lysine
-
 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [myo-inositol-1-phosphate synthetase]-L-lysine expression of Mycobacterium tuberculosis ino1, pafA and pupGlu, results in pupylated myo-inositol-1-phosphate synthetase (Ino1) in Escherichia coli Mycobacterium tuberculosis ATCC 25618 ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[myo-inositol-1-phosphate synthetase]-L-lysine
-
 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [phosphoenolpyruvate-protein phosphotransferase I]-L-lysine
-
 Mycobacterium tuberculosis ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[phosphoenolpyruvate-protein phosphotransferase I]-L-lysine
-
 ?
6.3.1.19 ATP + [prokaryotic ubiquitin-like protein]-L-glutamate + [phosphoenolpyruvate-protein phosphotransferase I]-L-lysine
-
 Mycobacterium tuberculosis ATCC 25618 ADP + phosphate + N6-([prokaryotic ubiquitin-like protein]-gamma-L-glutamyl)-[phosphoenolpyruvate-protein phosphotransferase I]-L-lysine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.19 PafA ambiguous Mycobacterium tuberculosis
6.3.1.19 proteasome accessory factor A
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 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
6.3.1.19 metabolism prokaryotic ubiquitin-like protein (Pup) is a post-translational modifier that attaches to more than 50 proteins in Mycobacteria. Proteasome accessory factor A (PafA) is responsible for Pup conjugation to substrates Mycobacterium tuberculosis