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Literature summary extracted from

  • Meguro, A.; Tomita, T.; Nishiyama, M.; Kuzuyama, T.
    Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton (2013), ChemBioChem, 14, 316-321.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.148 overexpression in Escherichia coli Streptomyces sp.
4.2.3.149 overexpression in Escherichia coli Streptomyces sp.
4.2.3.150 overexpression in Escherichia coli Streptomyces sp.
4.2.3.151 overexpression in Escherichia coli Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.148 0.0937
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.149 0.0421
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.149 0.0937
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.150 0.0421
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.151 0.0421
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.148 Mg2+ required Streptomyces sp.
4.2.3.149 Mg2+ required Streptomyces sp.
4.2.3.150 Mg2+ required Streptomyces sp.
4.2.3.151 Mg2+ required Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.148 42000
-
 2 * 42000, SDS-PAGE Streptomyces sp.
4.2.3.148 95000
-
 gel filtration Streptomyces sp.
4.2.3.149 38000
-
 2 * 38000, SDS-PAGE Streptomyces sp.
4.2.3.149 42000
-
 2 * 42000, SDS-PAGE Streptomyces sp.
4.2.3.149 69000
-
 gel filtration Streptomyces sp.
4.2.3.149 95000
-
 gel filtration Streptomyces sp.
4.2.3.150 38000
-
 2 * 38000, SDS-PAGE Streptomyces sp.
4.2.3.150 69000
-
 gel filtration Streptomyces sp.
4.2.3.151 38000
-
 2 * 38000, SDS-PAGE Streptomyces sp.
4.2.3.151 69000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.148 geranylgeranyl diphosphate Streptomyces sp.
-
 cembrene C + diphosphate cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene ?
4.2.3.148 geranylgeranyl diphosphate Streptomyces sp. SANK 60404
-
 cembrene C + diphosphate cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene ?
4.2.3.149 geranylgeranyl diphosphate Streptomyces sp.
-
 (R)-nephthenol + diphosphate
-
 ?
4.2.3.149 geranylgeranyl diphosphate Streptomyces sp. SANK 60404
-
 (R)-nephthenol + diphosphate
-
 ?
4.2.3.150 geranylgeranyl diphosphate Streptomyces sp.
-
 (R)-cembrene A + diphosphate
-
 ?
4.2.3.150 geranylgeranyl diphosphate Streptomyces sp. SANK 60404
-
 (R)-cembrene A + diphosphate
-
 ?
4.2.3.151 geranylgeranyl diphosphate Streptomyces sp.
-
 (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?
4.2.3.151 geranylgeranyl diphosphate Streptomyces sp. SANK 60404
-
 (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.148 Streptomyces sp. M1V9Q0
-
-
4.2.3.148 Streptomyces sp. SANK 60404 M1V9Q0
-
-
4.2.3.149 Streptomyces sp. M1V9Q0
-
-
4.2.3.149 Streptomyces sp. M1VDX3
-
-
4.2.3.149 Streptomyces sp. SANK 60404 M1V9Q0
-
-
4.2.3.149 Streptomyces sp. SANK 60404 M1VDX3
-
-
4.2.3.150 Streptomyces sp. M1VDX3
-
-
4.2.3.150 Streptomyces sp. SANK 60404 M1VDX3
-
-
4.2.3.151 Streptomyces sp. M1VDX3
-
-
4.2.3.151 Streptomyces sp. SANK 60404 M1VDX3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.148
-
 Streptomyces sp.
4.2.3.149
-
 Streptomyces sp.
4.2.3.150
-
 Streptomyces sp.
4.2.3.151
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.148 geranylgeranyl diphosphate
-
 Streptomyces sp. cembrene C + diphosphate cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene ?
4.2.3.148 geranylgeranyl diphosphate the bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase Streptomyces sp. cembrene C + diphosphate cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene ?
4.2.3.148 geranylgeranyl diphosphate
-
 Streptomyces sp. SANK 60404 cembrene C + diphosphate cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene ?
4.2.3.148 geranylgeranyl diphosphate the bifunctional enzyme also produces (R)-nephthenol. See EC 4.2.3.149, nephthenol synthase Streptomyces sp. SANK 60404 cembrene C + diphosphate cembrene C i.e. (1E,5E,9E)-1,5,9-trimethyl-12-(propan-2-ylidene)cyclotetradeca-1,5,9-triene ?
4.2.3.149 geranylgeranyl diphosphate
-
 Streptomyces sp. (R)-nephthenol + diphosphate
-
 ?
4.2.3.149 geranylgeranyl diphosphate the bifunctional enzyme also produces cembrene C (see EC 4.2.3.148, cembrene C synthase) Streptomyces sp. (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
4.2.3.149 geranylgeranyl diphosphate the enzyme also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase) Streptomyces sp. (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
4.2.3.149 geranylgeranyl diphosphate
-
 Streptomyces sp. SANK 60404 (R)-nephthenol + diphosphate
-
 ?
4.2.3.149 geranylgeranyl diphosphate the bifunctional enzyme also produces cembrene C (see EC 4.2.3.148, cembrene C synthase) Streptomyces sp. SANK 60404 (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
4.2.3.149 geranylgeranyl diphosphate the enzyme also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase) Streptomyces sp. SANK 60404 (R)-nephthenol + diphosphate (R)-nephthenol i.e. 2-[(1R,3E,7E,11E)-4,8,12-trimethyltetradeca-3,7,11-trien-1-yl]propan-2-ol ?
4.2.3.150 geranylgeranyl diphosphate
-
 Streptomyces sp. (R)-cembrene A + diphosphate
-
 ?
4.2.3.150 geranylgeranyl diphosphate the trifunctional enzyme also produces (R)-nephthenol and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol. See EC 4.2.3.149, nephthenol synthase and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase Streptomyces sp. (R)-cembrene A + diphosphate (R)-cembrene A i.e. (1E,5E,9E,12R)-1,5,9-trimethyl-12-(propan-2-en-2-yl)cyclotetradeca-1,5,9-triene ?
4.2.3.150 geranylgeranyl diphosphate
-
 Streptomyces sp. SANK 60404 (R)-cembrene A + diphosphate
-
 ?
4.2.3.150 geranylgeranyl diphosphate the trifunctional enzyme also produces (R)-nephthenol and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol. See EC 4.2.3.149, nephthenol synthase and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase Streptomyces sp. SANK 60404 (R)-cembrene A + diphosphate (R)-cembrene A i.e. (1E,5E,9E,12R)-1,5,9-trimethyl-12-(propan-2-en-2-yl)cyclotetradeca-1,5,9-triene ?
4.2.3.151 geranylgeranyl diphosphate
-
 Streptomyces sp. (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?
4.2.3.151 geranylgeranyl diphosphate the trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase Streptomyces sp. (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?
4.2.3.151 geranylgeranyl diphosphate
-
 Streptomyces sp. SANK 60404 (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.148 homodimer 2 * 42000, SDS-PAGE Streptomyces sp.
4.2.3.149 homodimer 2 * 38000, SDS-PAGE Streptomyces sp.
4.2.3.149 homodimer 2 * 42000, SDS-PAGE Streptomyces sp.
4.2.3.150 homodimer 2 * 38000, SDS-PAGE Streptomyces sp.
4.2.3.151 homodimer 2 * 38000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
4.2.3.148 DtcycA
-
 Streptomyces sp.
4.2.3.149 DtcycA gene name. Two isozymes with this activity are isolated from the bacterium Streptomyces sp. SANK 60404. The enzyme encoded by the DtcycA gene also produces cembrene C (see EC 4.2.3.148, cembrene C synthase), while the enzyme encoded by the DtcycB gene also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151 (pentamethylcyclopentadecatrienol synthase)) Streptomyces sp.
4.2.3.149 DtcycB gene name. The trifunctional enzyme also produces (R)-cembrene A and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol (see EC 4.2.3.150, cembrene A synthase, and EC 4.2.3.151 (pentamethylcyclopentadecatrienol synthase)) Streptomyces sp.
4.2.3.150 DtcycB gene name. The trifunctional enzyme also produces (R)-nephthenol and (1S,4E,8E,12E)-2,2,5,9,13-pentamethylcyclopentadeca-4,8,12-trien-1-ol. See EC 4.2.3.149, nephthenol synthase and EC 4.2.3.151, pentamethylcyclopentadecatrienol synthase Streptomyces sp.
4.2.3.151 DtcycB gene name. The trifunctional enzyme also produces (R)-nephthenol and (R)-cembrene A. See EC 4.2.3.150, cembrene A synthase and EC 4.2.3.149, nephthenol synthase Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.148 30
-
 assay at Streptomyces sp.
4.2.3.149 30
-
 assay at Streptomyces sp.
4.2.3.150 30
-
 assay at Streptomyces sp.
4.2.3.151 30
-
 assay at Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.148 0.047
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.149 0.022
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.149 0.047
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.150 0.022
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.
4.2.3.151 0.022
-
 geranylgeranyl diphosphate pH 7.5, 30°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.148 7.5
-
 assay Streptomyces sp.
4.2.3.149 7.5
-
 assay Streptomyces sp.
4.2.3.150 7.5
-
 assay Streptomyces sp.
4.2.3.151 7.5
-
 assay Streptomyces sp.