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Literature summary extracted from

  • Van Eldijk, M.; Pieters, B.; Mikhailov, V.; Robinson, C.; Van Hest, J.; Mecinovic, J.
    Catenane versus ring: do both assemblies of CS2 hydrolase exhibit the same stability and catalytic activity? (2014), Chem. Sci., 5, 2879-2884.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.1.5 additional information inability of the small KCN and CF3SO2NH2 to inhibit CS2 hydrolase Acidianus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.5 additional information
-
 additional information kinetics and thermodynamics, overview Acidianus sp.
3.13.1.5 0.178
-
 carbon disulfide pH 7.5, 50°C, ring form enzyme Acidianus sp.
3.13.1.5 0.193
-
 carbon disulfide pH 7.5, 50°C, catenane form enzyme Acidianus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.13.1.5 Zn2+ required Acidianus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.5 carbon disulfide + 2 H2O Acidianus sp.
-
 CO2 + 2 hydrogen sulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.5 Acidianus sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.5 carbon disulfide + 2 H2O
-
 Acidianus sp. CO2 + 2 hydrogen sulfide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.13.1.5 More the enzyme exists in solution as a mixture of unique hexadecameric catenane and octameric ring forms, multi-angle laser light scattering and native mass spectrometric analyses, overview. Four of the sixteen active sites of the catenane can be partially blocked due to the packed perpendicular arrangement of the two interlocked rings Acidianus sp.

Synonyms

EC Number Synonyms Comment Organism
3.13.1.5 CS2 hydrolase
-
 Acidianus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.1.5 50
-
 assay at Acidianus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13.1.5 48
-
 carbon disulfide pH 7.5, 50°C, catenane form enzyme Acidianus sp.
3.13.1.5 72
-
 carbon disulfide pH 7.5, 50°C, ring form enzyme Acidianus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.1.5 7.5
-
 assay at Acidianus sp.

General Information

EC Number General Information Comment Organism
3.13.1.5 additional information the ring form of CS2 hydrolase possesses higher enzyme efficiency (per monomer) than the catenane form, whereas the catenane form is overall more active (per assembly) Acidianus sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.13.1.5 249
-
 carbon disulfide pH 7.5, 50°C, catenane form enzyme Acidianus sp.
3.13.1.5 409
-
 carbon disulfide pH 7.5, 50°C, ring form enzyme Acidianus sp.