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Literature summary extracted from
- Clipping of predetermined fragments from the human genome by S1 nuclease-PNA combinations (2014), Chem. Commun. (Camb. ), 50, 8674-8676.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | molecular biology | a combination of S1 nuclease with two strands of pseudo-complementary peptide nucleic acid is useful for cleaving genomic DNA into desired fragments at targeted sites. The DNA analogue pseudo-complementary peptide nucleic acid, pcPNA, has a poly[N-aminoethylglycine] backbone and invades double-stranded DNA through Watson-Crick base-pairing. Through the invasion, single-stranded portions are formed at targeted sites in the genome and cut by the enzyme which hydrolyses only single-stranded DNA. The enzyme's mismatch-recognition activity was high enough to distinguish one base-pair difference in the invasion site. Enzyme cofactor Zn2+ suppresses the DNA invasion by pseudo-complementary peptide nucleic acid | Aspergillus oryzae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.30.1 | Zn2+ | required, divalent metal ion stabilizes the DNA duplex and suppresses the pcPNA invasion | Aspergillus oryzae |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | Aspergillus oryzae | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.30.1 | commercial preparation | - |
Aspergillus oryzae | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.30.1 | additional information | the enzyme cleaves human genomic DNA from the genome of K562 or HEK-293 cells at targeted sites by combining S1 nuclease with two strands of pseudo-complementary peptide nucleic acid, DNA fragment preparation, overview | Aspergillus oryzae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.30.1 | S1 nuclease | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 50 | - |
higher activity at 50°C compared to 37°C | Aspergillus oryzae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 37 | 50 | assay at | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.30.1 | 7 | - |
assay at | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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