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Literature summary extracted from

  • Reveal, B.S.; Paietta, J.V.
    Sulfur-regulated control of the met-2+ gene of Neurospora crassa encoding cystathionine beta-lyase (2013), BMC Res. Notes, 6, 259.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.13 CYS3 a bZIP transcriptional activator, is the key regulator and necessary for regulation expression of the sulfur-related genes, binds on a consensus sequence for CYS3 binding Neurospora crassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 gene met-2+, DNA and amino acid sequence determination and analysis, presence of CYS3 activator binding sites on the met-2+ promoter, quantitative enzyme expression analysis Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 L-cystathionine + H2O Neurospora crassa
-
 L-homocysteine + pyruvate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Neurospora crassa Q1K8G0 gene met-2+
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystathionine + H2O
-
 Neurospora crassa L-homocysteine + pyruvate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.13 beta-cystathionase
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate
-
 Neurospora crassa

Expression

EC Number Organism Comment Expression
4.4.1.13 Neurospora crassa in a activator knockout DELTAcys-3 mutant strain, the met-2+ transcript levels are substantially reduced under either low- or high-sulfur growth conditions down
4.4.1.13 Neurospora crassa expression of met-2+ in wild-type Neurspora crassa increases 3.1fold under sulfur-limiting growth conditions as compared to the transcript levels seen under high sulfur growth conditions, i.e., repressing conditions up

General Information

EC Number General Information Comment Organism
4.4.1.13 metabolism cystathionine beta-lyase is part of the CYS3-controlled regulatory network Neurospora crassa
4.4.1.13 physiological function cystathionine beta-lyase performs an essential role in the transsulfuration pathway by its primary reaction of forming homocysteine from cystathionine. It allows for the utilization of the intracellular pool of cystathionine for the synthesis of homocysteine which serves as the immediate precursor to methionine Neurospora crassa