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Literature summary extracted from

  • Ueda, S.; Nomoto, R.; Yoshida, K.; Osawa, R.
    Comparison of three tannases cloned from closely related Lactobacillus species: L. plantarum, L. paraplantarum, and L. pentosus (2014), BMC Microbiol., 14, 87.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 EDTA 103.7% activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 Urea 119.5% activity at 1 mM Lactiplantibacillus pentosus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.20 expressed in in Bacillus subtilis strain RIK 1285 Lactiplantibacillus pentosus
3.1.1.20 expressed in in Bacillus subtilis strain RIK 1285 Lactiplantibacillus plantarum
3.1.1.20 expressed in in Bacillus subtilis strain RIK 1285 Lactiplantibacillus paraplantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 Ca2+ 88.7% residual activity at 1 mM Lactiplantibacillus paraplantarum
3.1.1.20 Ca2+ 92.3% residual activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 Ca2+ 98.3% residual activity at 1 mM Lactiplantibacillus plantarum
3.1.1.20 EDTA the enzyme is partially inhibited by but not significantly affected (87.8% residual activity at 1 mM) Lactiplantibacillus paraplantarum
3.1.1.20 EDTA the enzyme is partially inhibited by but not significantly affected (97.6% residual activity at 1 mM) Lactiplantibacillus plantarum
3.1.1.20 Fe2+ 24.1% residual activity at 1 mM Lactiplantibacillus paraplantarum
3.1.1.20 Fe2+ 23.4% residual activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 Fe2+ 22.5% residual activity at 1 mM Lactiplantibacillus plantarum
3.1.1.20 Mg2+ 96.7% residual activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 Mn2+ 75.6% residual activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 Mn2+ 87.6% residual activity at 1 mM Lactiplantibacillus plantarum
3.1.1.20 phenylmethylsulfonyl fluoride the enzyme is partially inhibited by but not significantly affected (66.2% residual activity at 1 mM) Lactiplantibacillus paraplantarum
3.1.1.20 phenylmethylsulfonyl fluoride 81.2% residual activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 phenylmethylsulfonyl fluoride the enzyme is partially inhibited by but not significantly affected (83.2% residual activity at 1 mM) Lactiplantibacillus plantarum
3.1.1.20 Urea the enzyme is partially inhibited by but not significantly affected (96.9% residual activity at 1 mM) Lactiplantibacillus paraplantarum
3.1.1.20 Urea the enzyme is partially inhibited by but not significantly affected (91.4% residual activity at 1 mM) Lactiplantibacillus plantarum
3.1.1.20 Zn2+ 95.4% residual activity at 1 mM Lactiplantibacillus paraplantarum
3.1.1.20 Zn2+ 25.2% residual activity at 1 mM Lactiplantibacillus pentosus
3.1.1.20 Zn2+ 46.1% residual activity at 1 mM Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.005
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.03
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.03
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.04
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.04
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.05
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.05
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.05
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.06
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.06
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.06
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.06
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.07
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.08
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.1
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.37
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.5
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.87
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Mg2+ 110.5% activity at 1 mM Lactiplantibacillus paraplantarum
3.1.1.20 Mg2+ 123.7% activity at 1 mM Lactiplantibacillus plantarum
3.1.1.20 Mn2+ 111.3% activity at 1 mM Lactiplantibacillus paraplantarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.20 50000
-
 x * 50000, SDS-PAGE Lactiplantibacillus pentosus
3.1.1.20 50000
-
 x * 50000, SDS-PAGE Lactiplantibacillus plantarum
3.1.1.20 50000
-
 x * 50000, SDS-PAGE Lactiplantibacillus paraplantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Lactiplantibacillus pentosus
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Lactiplantibacillus plantarum
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Lactiplantibacillus paraplantarum
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Lactiplantibacillus pentosus 21A-3
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Lactiplantibacillus paraplantarum NSO120
-
 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O Lactiplantibacillus plantarum ATCC 14917T
-
 gallate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Lactiplantibacillus paraplantarum
-
-
-
3.1.1.20 Lactiplantibacillus paraplantarum NSO120
-
-
-
3.1.1.20 Lactiplantibacillus pentosus
-
-
-
3.1.1.20 Lactiplantibacillus pentosus 21A-3
-
-
-
3.1.1.20 Lactiplantibacillus plantarum B3Y018
-
-
3.1.1.20 Lactiplantibacillus plantarum ATCC 14917T B3Y018
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 TALON metal affinity resin column chromatography Lactiplantibacillus pentosus
3.1.1.20 TALON metal affinity resin column chromatography Lactiplantibacillus plantarum
3.1.1.20 TALON metal affinity resin column chromatography Lactiplantibacillus paraplantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 catechin gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
3.1.1.20 catechin gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
3.1.1.20 catechin gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
3.1.1.20 catechin gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 ?
-
 ?
3.1.1.20 catechin gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 ?
-
 ?
3.1.1.20 catechin gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ?
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 ?
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 ?
-
 ?
3.1.1.20 epicatechin gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T ?
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Lactiplantibacillus pentosus gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T gallate + epigallocatechin
-
 ?
3.1.1.20 epigallocatechin-3-O-(3-O-methyl) gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
3.1.1.20 epigallocatechin-3-O-(3-O-methyl) gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
3.1.1.20 epigallocatechin-3-O-(3-O-methyl) gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
3.1.1.20 gallocatechin gallate + H2O
-
 Lactiplantibacillus pentosus ?
-
 ?
3.1.1.20 gallocatechin gallate + H2O
-
 Lactiplantibacillus plantarum ?
-
 ?
3.1.1.20 gallocatechin gallate + H2O
-
 Lactiplantibacillus paraplantarum ?
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Lactiplantibacillus pentosus gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Lactiplantibacillus plantarum gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Lactiplantibacillus paraplantarum gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Lactiplantibacillus pentosus 21A-3 gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Lactiplantibacillus paraplantarum NSO120 gallate + methanol
-
 ?
3.1.1.20 methyl gallate + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T gallate + methanol
-
 ?
3.1.1.20 tannic acid + H2O
-
 Lactiplantibacillus pentosus gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Lactiplantibacillus plantarum gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Lactiplantibacillus paraplantarum gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Lactiplantibacillus pentosus 21A-3 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Lactiplantibacillus paraplantarum NSO120 gallate + D-glucose
-
 ?
3.1.1.20 tannic acid + H2O
-
 Lactiplantibacillus plantarum ATCC 14917T gallate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.20 ? x * 50000, SDS-PAGE Lactiplantibacillus pentosus
3.1.1.20 ? x * 50000, SDS-PAGE Lactiplantibacillus plantarum
3.1.1.20 ? x * 50000, SDS-PAGE Lactiplantibacillus paraplantarum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.20 tannin acyl hydrolase
-
 Lactiplantibacillus pentosus
3.1.1.20 tannin acyl hydrolase
-
 Lactiplantibacillus plantarum
3.1.1.20 tannin acyl hydrolase
-
 Lactiplantibacillus paraplantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 40
-
-
 Lactiplantibacillus pentosus
3.1.1.20 40
-
-
 Lactiplantibacillus plantarum
3.1.1.20 40
-
-
 Lactiplantibacillus paraplantarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.20 0.06
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.26
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 0.35
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 0.42
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.44
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 0.89
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 1.12
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 1.12
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 1.48
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 1.49
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 2.41
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 8.1
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 9.2
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 11.08
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 14.29
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 15.95
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 46.02
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 72.73
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 8.5
-
-
 Lactiplantibacillus pentosus
3.1.1.20 8.5
-
-
 Lactiplantibacillus plantarum
3.1.1.20 8.5
-
-
 Lactiplantibacillus paraplantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 8 10 the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 Lactiplantibacillus pentosus
3.1.1.20 8 10 the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 Lactiplantibacillus plantarum
3.1.1.20 8 10 the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 Lactiplantibacillus paraplantarum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.20 6.04
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 7.25
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 8.63
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 9.02
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 10.57
-
 epigallocatechin-3-O-(3-O-methyl) gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 11.68
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 14.32
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 18.79
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 19.22
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus pentosus
3.1.1.20 27.19
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 52.23
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 53.65
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 125
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus plantarum
3.1.1.20 145.1
-
 methyl gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 154.7
-
 gallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 181.5
-
 catechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 195.3
-
 epicatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum
3.1.1.20 260.8
-
 epigallocatechin gallate in 50 mM Tris-HCl (pH 8.0), at 37°C Lactiplantibacillus paraplantarum